Guangdong Provincial Key Laboratory of Marine Biotechnology, STU-UNIVPM Joint Algal Research Center, Department of Biology, College of Science, Shantou University, Shantou, Guangdong, PR China.
Crit Rev Food Sci Nutr. 2019;59(19):3165-3178. doi: 10.1080/10408398.2018.1486283. Epub 2018 Jul 16.
Every individual needs food for its nutritional value and flavor while the economic growth of a nation depends on a thriving profit-generating industry. The food industry caters to both needs in an efficient manner. Proteins can rightly be considered as the driving force behind the overwhelming success of this industry. However, purification of proteins is not an easy undertaking due to their intricate nature while presently employed procedures for this purpose, regrettably, are both costly, and labor- and time-intensive in addition to being unsettling on proteins structural conformity. ATPS has accumulated a lot of interest from the scientific community due to its mild operating conditions, high recovery yield, ease of scaling it up, and its cost-effective and environment friendly nature. This review tries to amass some accounts concerning the utility of ATPS for the separation and purification of proteins. Some auspicious clues in this regard can be witnessed along with a few loopholes which need to be addressed before this technique can truly demonstrate its potential industrial protein purification. Overall, a polymer - salt (citrates in particular) ATPS with an added inert supplementary salt can be regarded as a better option for purifying proteins.
每个人都需要食物来获取其营养价值和口感，而国家的经济增长则依赖于蓬勃发展的盈利产业。食品工业以高效的方式满足了这两方面的需求。蛋白质可以说是这个行业取得巨大成功的驱动力。然而，由于其复杂的性质，蛋白质的纯化并非易事，而目前用于此目的的程序不仅成本高、劳动和时间密集，而且还会破坏蛋白质的结构一致性。由于其温和的操作条件、高回收率、易于放大以及具有成本效益和环境友好的特点，ATPS 引起了科学界的广泛关注。这篇综述试图收集一些关于 ATPS 在蛋白质分离和纯化方面应用的资料。在这方面可以看到一些有希望的线索，以及在这项技术真正展示其潜在的工业蛋白质纯化能力之前需要解决的一些缺陷。总的来说，聚合物-盐（特别是柠檬酸盐）ATPS 加上惰性辅助盐可以被认为是一种更好的蛋白质纯化选择。
