Department of Biotechnology, Manipal institute of technology (MIT), Manipal Academy of Higher Education (MAHE), Manipal 576104, Karnataka, India.
Department of Chemical engineering, Manipal institute of technology (MIT), Manipal Academy of Higher Education (MAHE), Manipal 576104, Karnataka, India.
Sci Total Environ. 2021 Jul 15;778:146293. doi: 10.1016/j.scitotenv.2021.146293. Epub 2021 Mar 8.
Aqueous two-phase system (ATPS) is long seen as a technique that promotes higher purity and yield in a single step. It is witnessing increased acceptance as a preferred choice for combined goals of concentration, separation and purification of a target product, be it industrially valuable or environmentally contaminating. Purification of biomolecules like enzymes, proteins, nucleic acids, viruses, etc. has been the forte of ATPS. Currently, the technique is used for concentrating the toxic fractions from diverse industrial let offs, from food, dairy, beverage, pharmaceuticals, agriculture, dyeing, tannery and metal-processing industries. Apart from being simple, efficient, rapid, flexible, economical, and biocompatible, the selectivity, purity and yield are on par and sometimes higher than the traditional downstream steps. From an industrial angle too, problems related to scale-up of ATPS are being actively addressed. Many novel approaches are being added by way of varying ATPS components to increase the yield and purity. Another case in point is the inclusion of optimization techniques for zeroing in on the precise setting of the operating parameters. With increasing impetus to the approach, we attempt to draw attention from academia and industries, alike, that are developing novel tweaks to the currently existing practices in ATPS. This review aims to assess and evaluate the different types of ATPS that have been used for the recovery of valuables and contaminants from industrial waste discharges.
双水相体系（ATPS）长期以来被视为一种可在单一步骤中提高纯度和收率的技术。它作为一种用于集中、分离和纯化目标产物（无论是工业上有价值的还是环境上有污染的）的首选方法，其应用越来越受到认可。酶、蛋白质、核酸、病毒等生物分子的纯化一直是 ATPS 的强项。目前，该技术用于浓缩来自各种工业排放物（食品、乳制品、饮料、制药、农业、染色、制革和金属加工行业）的有毒部分。除了简单、高效、快速、灵活、经济和生物相容性外，其选择性、纯度和收率与传统的下游步骤相当，有时甚至更高。从工业角度来看，也在积极解决 ATPS 放大的问题。通过改变 ATPS 组件来增加产量和提高纯度的许多新方法正在被添加。另一个例子是包括优化技术，以确定操作参数的精确设置。随着该方法的不断推进，我们试图引起学术界和工业界的关注，他们正在对目前 ATPS 中的现有实践进行新的调整。本综述旨在评估和评估已经用于从工业废水中回收有价值物质和污染物的不同类型的 ATPS。
