McCarty D J, Cheung H S
Ann Rheum Dis. 1985 May;44(5):316-20. doi: 10.1136/ard.44.5.316.
Immunoreactive prostaglandin (PG) E2 was released into the ambient medium in a dose dependent fashion when either hydroxyapatite (HA) or calcium pyrophosphate dihydrate (CPPD) crystals were added to canine synovial fibroblasts in tissue culture. PGE2 release peaked 6 to 9 hours after HA or CPPD crystals were added in the presence of serum but at 24 hours if they were added in the presence of lactalbumin hydrolysate. PGE2 release correlated with crystal endocytosis estimated qualitatively by serial phase contrast microscopy and time lapse photography. As postulated previously by others for monosodium urate crystals, prostaglandin production by synovial cells may also be related to the pathogenesis of the destructive arthropathies associated with HA or CPPD crystals.
当在组织培养中将羟基磷灰石(HA)或二水焦磷酸钙(CPPD)晶体添加到犬滑膜成纤维细胞中时,免疫反应性前列腺素(PG)E2以剂量依赖性方式释放到周围培养基中。在有血清存在的情况下,添加HA或CPPD晶体后6至9小时PGE2释放达到峰值,但如果在乳白蛋白水解物存在的情况下添加,则在24小时达到峰值。PGE2释放与通过连续相差显微镜和延时摄影定性估计的晶体胞吞作用相关。正如其他人先前对尿酸钠晶体所假设的那样,滑膜细胞产生前列腺素也可能与HA或CPPD晶体相关的破坏性关节病的发病机制有关。