通过内吞作用刺激兔滑膜成纤维细胞分泌胶原酶和中性蛋白酶。
Stimulation by endocytosis of the secretion of collagenase and neutral proteinase from rabbit synovial fibroblasts.
作者信息
Werb Z, Reynolds J J
出版信息
J Exp Med. 1974 Dec 1;140(6):1482-97. doi: 10.1084/jem.140.6.1482.
Abstract
Rabbit synovial fibroblasts in monolayer culture secrete a specific collagenase and a neutral endopeptidase into their serum-free culture medium. The rate of secretion of these two enzymes is increased after the ingestion and storage of latex particles within the vacuolar system of the cells. The increased rates of secretion of the neutral enzymes are stable for over 2 wk in the absence of a further phagocytic bout. In constrast there is little change in the extracellular levels of two lysosomal hydrolases, cathepsin D and beta-glucuronidase. The increase in the secretory rates for the two neutral enzymes is related to the number of latex particles ingested by the cells, and increases of up to 12-fold over the nonphagocytosing cultures were observed. A variety of other materials including mycostatin particles and dextran sulfate also induced increases in the secretion of collagenase. These results are discussed in relation to the turnover of connective tissue matrix macromolecules.
摘要
单层培养的兔滑膜成纤维细胞向其无血清培养基中分泌一种特异性胶原酶和一种中性内肽酶。在细胞液泡系统内摄取和储存乳胶颗粒后，这两种酶的分泌速率增加。在没有进一步吞噬活动的情况下，中性酶分泌速率的增加在超过2周的时间内保持稳定。相比之下，两种溶酶体水解酶，组织蛋白酶D和β-葡萄糖醛酸酶的细胞外水平几乎没有变化。两种中性酶分泌速率的增加与细胞摄取的乳胶颗粒数量有关，并且观察到与非吞噬培养相比增加高达12倍。包括制霉菌素颗粒和硫酸葡聚糖在内的多种其他物质也诱导胶原酶分泌增加。本文就这些结果与结缔组织基质大分子的更新进行了讨论。
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