Department of Biotechnology, Chandigarh College of Technology, CGC Landran, Mohali, Punjab, 140307, India.
Department of Biotechnology, Chandigarh College of Technology, CGC Landran, Mohali, Punjab, 140307, India.
Int J Biol Macromol. 2018 Oct 1;117:1093-1100. doi: 10.1016/j.ijbiomac.2018.06.007. Epub 2018 Jun 7.
Decolourization of textile effluent is always being a problem of major concern for the safe disposal of effluent water in river stream. Due to intense colour of the effluent water, even after the treatment, disposal of the water in river stream is under strict environmental regulation. Laccase, a natural oxidase, have the miraculous power to decolourize different dyes with or without mediators. However, stability and cost of implementation of the enzyme at industrial conditions make the entire process unfeasible at industrial scale. In the present study, a highly thermo-alkali-stable, metal, surfactant and organic solvent tolerant laccase from Bacillus sp. MSK-01 was immobilized in Cu-alginate bead with ABTS mediator under standardized conditions. A continuous flow packed bed bioreactor was formulated to develop a continuous method of the treatment of effluent water. Results showed 66% reduction in the colour of the effluent. UV-VIS spectrum analysis of the treated and untreated samples showed the formation of the degradation products of dyes due to the action of laccase. The developed process can be made useful for on site industrial application.
纺织废水的脱色一直是河流中废水安全处理的主要关注点。由于废水的颜色很深，即使经过处理，也需要严格按照环境规定才能将水排入河流。漆酶作为一种天然氧化酶，具有神奇的能力，可以在有或没有介体的情况下对不同的染料进行脱色。然而，酶在工业条件下的稳定性和成本使得整个过程在工业规模上不可行。在本研究中，从芽孢杆菌 MSK-01 中分离出一种高度耐热、耐碱、耐金属、耐表面活性剂和有机溶剂的漆酶，并用 ABTS 介体制成海藻酸钙珠进行固定化。设计了一个连续流填充床生物反应器，以开发一种连续处理废水的方法。结果表明，废水的颜色降低了 66%。对处理前后样品的 UV-VIS 光谱分析表明，由于漆酶的作用，染料的降解产物形成。开发的工艺可用于现场工业应用。
