Ecological Security and Protection Key Laboratory of Sichuan Province, Mianyang Normal University, Mianyang, China.
State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China; Department of Microbiology, The University of Hong Kong, Hong Kong, China; Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China.
Virology. 2018 Aug;521:77-91. doi: 10.1016/j.virol.2018.05.027. Epub 2018 Jun 7.
The Sichuan takin inhabits the bamboo forests in the Eastern Himalayas and is considered as a national treasure of China with the highest legal protection and conservation status considered as vulnerable according to The IUCN Red List of Threatened Species. In this study, fecal samples of 71 Sichuan takins were pooled and deep sequenced. Among the 103,553 viral sequences, 21,961 were assigned to mammalian viruses. De novo assembly revealed genomes of an enterovirus and an astrovirus and contigs of circoviruses and genogroup I picobirnaviruses. Complete genome sequencing and phylogenetic analysis showed that Sichuan takin enterovirus is a novel serotype/genotype of the species Enterovirus G, with evidence of recombination. Sichuan takin astrovirus is a new subtype of bovine astrovirus, probably belonging to a new genogroup in the genus Mamastrovirus. Further studies will reveal whether these viruses can also be found in Mishmi takin and Shaanxi takin and their pathogenic potentials.
四川羚牛栖息于东喜马拉雅山的竹林中，被视为中国的国宝，受到最高法律保护，其保护状况被视为易危，按照世界自然保护联盟濒危物种红色名录的标准。本研究中，对 71 份四川羚牛粪便样本进行了合并深度测序。在 103553 条病毒序列中，有 21961 条被分配给哺乳动物病毒。从头组装揭示了肠病毒和星状病毒的基因组以及圆环病毒和 I 组禽双 RNA 病毒的基因片段。完整基因组测序和系统发育分析表明，四川羚牛肠病毒是肠病毒 G 种的一个新血清型/基因型，存在重组证据。四川羚牛星状病毒是牛星状病毒的一个新亚型，可能属于 Mamastrovirus 属的一个新基因群。进一步的研究将揭示这些病毒是否也存在于羚牛和陕西羚牛中，以及它们的致病潜力。
