Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
Laboratory for the Prevention and Control of Bovine and Goat Diseases, College of Animal Science and Technology, Guangxi Vocational University of Agriculture, Nanning 530007, China.
Viruses. 2024 Jul 29;16(8):1213. doi: 10.3390/v16081213.
Astroviruses are single-stranded, positive-sense RNA viruses capable of infecting humans as well as a wide range of mammalian and avian species, with a length of approximately 6.6-7.7 kb. In this study, 139 goat fecal samples collected from the Guangxi province were used for the RT-PCR detection, and two of these were positive for goat astrovirus, with a positivity rate of 1.44% (2/139). The complete genome sequence of an astrovirus strain and the partial genome sequence of a strain astrovirus, named GX WZ 2023 and GX HC 2023, were amplified and sequenced, and their sequence lengths were 6284 nt and 6213 nt, respectively. Among them, the capsid protein of goat astrovirus GX HC 2023 showed the highest amino acid identity of 95.9% with ovine astrovirus GX, which belonged to the MAstV-2 genotype. However, the closest relative of the GX WZ 2023 strain was found to be the caprine astrovirus Sichuan, with a nucleotide sequence identity of 76.8%. The ORF1ab nonstructural protein of this strain showed the highest amino acid identities of 89.2 and 95.8% with the ovine astrovirus S5.1 and caprine astrovirus G5.1 strains, respectively. However, its ORF2 capsid protein has 68.4% amino acid identity with the bovine astrovirus (BAstV) 16 2021 CHN strain and only 21.9-64% amino acid identity with all available strains of goat astrovirus. The GX WZ 2023 strain was recombined with the Chinese (BAstV 16 2021 CHN) and Japanese bovine strains (BAstV JPN 2015) in the ORF2 region. Therefore, the goat astrovirus GX WZ 2023 is proposed as a new member of the family goat astroviridae based on the species classification criteria of the International Committee on Taxonomy of Viruses. These findings enhance our understanding of the prevalence and genetic evolution of goat astrovirus and provide a scientific basis for future studies of these viruses in other animals.
星状病毒是一种单链、正链 RNA 病毒，能够感染人类以及广泛的哺乳动物和禽类，其长度约为 6.6-7.7kb。在本研究中，使用 RT-PCR 检测了从广西省采集的 139 份山羊粪便样本，其中 2 份为山羊星状病毒阳性，阳性率为 1.44%（2/139）。扩增并测序了一株星状病毒株和一株星状病毒株的部分基因组序列，分别命名为 GX WZ 2023 和 GX HC 2023，其序列长度分别为 6284nt 和 6213nt。其中，山羊星状病毒 GX HC 2023 的衣壳蛋白与绵羊星状病毒 GX 的氨基酸同源性最高，为 95.9%，属于 MAstV-2 基因型。然而，GX WZ 2023 株与四川山羊星状病毒的亲缘关系最近，核苷酸序列同源性为 76.8%。该株的 ORF1ab 非结构蛋白与绵羊星状病毒 S5.1 和山羊星状病毒 G5.1 株的氨基酸同源性最高，分别为 89.2%和 95.8%。然而，其 ORF2 衣壳蛋白与牛星状病毒（BAstV）16 2021 CHN 株的氨基酸同源性为 68.4%，与所有可用的山羊星状病毒株的氨基酸同源性仅为 21.9-64%。GX WZ 2023 株在 ORF2 区与中国（BAstV 16 2021 CHN）和日本牛株（BAstV JPN 2015）重组。因此，根据国际病毒分类委员会的物种分类标准，提出该山羊星状病毒 GX WZ 2023 为星状病毒科的一个新成员。这些发现增强了我们对山羊星状病毒流行和遗传进化的认识，为今后在其他动物中研究这些病毒提供了科学依据。
