Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture, Nanjing 210014, China.
Animal Science College, Tibet Agriculture and Animal Husbandry University, Nyingchi 860000, China.
Viruses. 2021 Sep 23;13(10):1907. doi: 10.3390/v13101907.
Calf diarrhea is one of the common diseases involved in the process of calf feeding. In this study, a sample of calf diarrhea that tested positive for bovine coronavirus and bovine astrovirus was subjected to high-throughput sequencing. The reassembly revealed the complete genomes of bovine norovirus, bovine astrovirus, bovine kobuvirus, and the S gene of bovine coronavirus. Phylogenetic analysis showed that the ORF2 region of bovine astrovirus had the lowest similarity with other strains and gathered in the Mamastrovirus unclassified genogroup, suggesting a new serotype/genotype could appear. Compared with the most closely related strain, there are six amino acid mutation sites in the S gene of bovine coronavirus, most of which are located in the S1 subunit region. The bovine norovirus identified in our study was BNoV-GIII 2, based on the VP1 sequences. The bovine kobuvirus is distributed in the Aichi virus B genus; the P1 gene shows as highly variable, while the 3D gene is highly conserved. These findings enriched our knowledge of the viruses in the role of calf diarrhea, and help to develop an effective strategy for disease prevention and control.
犊牛腹泻是犊牛饲养过程中常见的疾病之一。本研究对检测出牛冠状病毒和牛星状病毒阳性的犊牛腹泻样本进行高通量测序。重新组装揭示了牛诺如病毒、牛星状病毒、牛博卡病毒和牛冠状病毒 S 基因的完整基因组。系统进化分析显示，牛星状病毒的 ORF2 区与其他毒株的相似度最低，聚集在未分类的 Mamastrovirus 属基因群中，提示可能出现新的血清型/基因型。与最密切相关的毒株相比，牛冠状病毒 S 基因有 6 个氨基酸突变位点，大多数位于 S1 亚单位区域。本研究鉴定的牛诺如病毒基于 VP1 序列，属于 BNoV-GIII 2。牛博卡病毒分布在 Aichi 病毒 B 属；P1 基因高度可变，而 3D 基因高度保守。这些发现丰富了我们对犊牛腹泻相关病毒的认识，有助于制定有效的疾病防控策略。
