Farndale R W, Murray J C
Calcif Tissue Int. 1985 Mar;37(2):178-82. doi: 10.1007/BF02554838.
Primary and passaged cultures of fibroblasts (RBMFs) raised from the bone marrow stroma of young rabbits were treated with pulsed electromagnetic fields (PEMFs) from the start of each culture until 1 week after they became confluent. the PEMF treatment had no effect on cell proliferation, estimated by phase contrast microscopy, by 3H-thymidine incorporation into DNA, or by total DNA assay. Collagen production, estimated by conversion of 3H-proline to 3H-hydroxyproline in nondialyzable material was markedly elevated in postconfluent cultures, but not in cultures that had only just reached confluence. About 65 of 3H-hydroxyproline was in low molecular weight form, and a correlation between collagen breakdown and cyclic AMP (cAMP) levels in RBMFs was demonstrated by adding dibutyryl cAMP or prostaglandin E3 (PGE2) to the culture medium concurrently with 3H-proline. The PEMF apparatus caused an insufficient temperature rise (less than 0.1 degree C) to account for these results. We propose that the rise in collagen production is consistent with the hypothesis that PEMFs act by reducing cAMP levels in RBMFs, and that thermal effects are insignificant.
从幼兔骨髓基质中培养出的原代和成代培养的成纤维细胞（RBMFs），从每种培养开始直至汇合后1周，均用脉冲电磁场（PEMFs）处理。通过相差显微镜、3H-胸腺嘧啶核苷掺入DNA或总DNA测定法估计，PEMF处理对细胞增殖没有影响。通过在不可透析物质中3H-脯氨酸转化为3H-羟脯氨酸来估计，汇合后培养物中的胶原蛋白产量显著升高，但刚达到汇合的培养物中则没有升高。约65%的3H-羟脯氨酸呈低分子量形式，通过在添加3H-脯氨酸的同时向培养基中添加二丁酰cAMP或前列腺素E3（PGE2），证明了RBMFs中胶原蛋白分解与环磷酸腺苷（cAMP）水平之间的相关性。PEMF装置引起的温度升高不足（低于0.1摄氏度），无法解释这些结果。我们提出，胶原蛋白产量的增加与PEMFs通过降低RBMFs中的cAMP水平起作用的假设一致，并且热效应不显著。
