Christman M F, Morgan R W, Jacobson F S, Ames B N
Cell. 1985 Jul;41(3):753-62. doi: 10.1016/s0092-8674(85)80056-8.
S. typhimurium become resistant to killing by hydrogen peroxide and other oxidants when pretreated with nonlethal levels of hydrogen peroxide. During adaptation to hydrogen peroxide, 30 proteins are induced. Nine are constitutively overexpressed in dominant hydrogen peroxide-resistant oxyR mutants. Mutant oxyR1 is resistant to a variety of oxidizing agents and overexpresses at least five enzyme activities involved in defenses against oxidative damage. Deletions of oxyR are recessive and uninducible by hydrogen peroxide for the nine proteins overexpressed in oxyR1, demonstrating that oxyR is a positive regulatory element. The oxyR1 mutant is also more resistant than the wild-type parent to killing by heat, and it constitutively overexpresses three heat-shock proteins. The oxyR regulatory network is a previously uncharacterized global regulatory system in enteric bacteria.
鼠伤寒沙门氏菌在用非致死剂量的过氧化氢预处理后，会对过氧化氢和其他氧化剂的杀伤产生抗性。在适应过氧化氢的过程中，会诱导产生30种蛋白质。在显性过氧化氢抗性oxyR突变体中，有9种蛋白质组成型过表达。突变体oxyR1对多种氧化剂具有抗性，并且至少过表达五种参与抗氧化损伤防御的酶活性。oxyR的缺失是隐性的，并且对于oxyR1中过表达的9种蛋白质，过氧化氢无法诱导其表达，这表明oxyR是一种正向调节元件。oxyR1突变体也比野生型亲本更耐热，并且组成型过表达三种热休克蛋白。oxyR调控网络是肠道细菌中一个以前未被表征的全局调控系统。
