Khomarlou Negin, Aberoomand-Azar Parviz, Lashgari Ardalan Pasdaran, Tebyanian Hamid, Hakakian Ali, Ranjbar Reza, Ayatollahi Seyed Abdolmajid
1 Department of Chemistry, Science and Research Branch, Islamic Azad University , Tehran , Iran.
2 Medicinal Plants Processing Research Center, Shiraz University of Medicinal Sciences , Shiraz , Iran.
Acta Biol Hung. 2018 Jun;69(2):144-155. doi: 10.1556/018.69.2018.2.4.
The objective of this study was to identify the bioactive compounds of essential oil and evaluate the antibacterial activity of the essential oil extracted from Chenopodium album subsp. striatum against multidrug-resistant bacterial strains (MDR) which were isolated from clinical specimens by conventional methods. Furthermore, eight different Gram-negative and Gram-positive multidrug-resistant bacterial strains were used to investigate the antibacterial potential of the essential oil. The antibacterial activity was tested using MIC and MBC microdilution method, well and disc diffusion in different concentration. The hydro-distillation of aerial parts powder yield was 0.466% (v/w). Essential oil showed bactericidal activity against both MDR Gram-negative and Gram-positive bacterial strains. MIC and MBC results were ranged from 0.31 to 2.5 and 0.62 to 5.0 mg/mL. The inhibition zones in well-diffusion method were ranged from 7 ± 0.6 mm to 15 ± 1.0 mm. Disc diffusion method was ranged from 7 ± 0.0 mm to 16 ± 0.6 mm depending on the type of bacteria strain and essential oil concentration. Essential oil of Ch. album had the greatest potential to be considered as an antibacterial agent against MDR bacteria strain. This potential was due to different biological and bioactive compounds like phytol, linalool, α-terpineol and linolenic acid in the plant.
本研究的目的是鉴定精油中的生物活性化合物，并评估从藜（Chenopodium album subsp. striatum）中提取的精油对通过常规方法从临床标本中分离出的多药耐药细菌菌株（MDR）的抗菌活性。此外，使用八种不同的革兰氏阴性和革兰氏阳性多药耐药细菌菌株来研究该精油的抗菌潜力。采用MIC和MBC微量稀释法、不同浓度的孔板扩散法和纸片扩散法测试抗菌活性。地上部分粉末的水蒸馏产率为0.466%（v/w）。精油对多药耐药革兰氏阴性和革兰氏阳性细菌菌株均表现出杀菌活性。MIC和MBC结果范围分别为0.31至2.5mg/mL和0.62至5.0mg/mL。孔板扩散法中的抑菌圈范围为7±0.6mm至15±1.0mm。纸片扩散法的抑菌圈范围为7±0.0mm至16±0.6mm，这取决于细菌菌株类型和精油浓度。藜的精油最有潜力被视为针对多药耐药细菌菌株的抗菌剂。这种潜力归因于植物中不同的生物和生物活性化合物，如叶绿醇、芳樟醇、α-松油醇和亚麻酸。
