State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China.
Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611730, China.
Molecules. 2018 Jun 8;23(6):1391. doi: 10.3390/molecules23061391.
has been used as an ethnomedicine for lowering blood sugar. To clarify the bioactive chemical constituents contributing to lower blood sugar, chemical investigation on the fruiting bodies of was conducted by chromatographic techniques, and led to the isolation of 14 compounds. Their structures were elucidated as triterpenoid lactones (⁻ and ) and ganoderma acids (⁻ and ⁻) based on the analysis of extensive spectroscopy (mass spectrometry (MS), nuclear magnetic resonance (NMR), infrared (IR), and ultraviolet (UV)) and comparison with literature data. Compounds , , , and ⁻ were evaluated for -glucosidase inhibitory activity. Compounds ⁻ are new compounds. Compounds ⁻ and were characteristic of an oxaspirolactone moiety, consisting of a five-membered ether ring, a five-membered lactone ring, and a characteristic C-23 spiro carbon. It is rare for natural products that such an oxaspirolactone moiety occurred in the lanostane-type triterpenoids. Compounds ⁻ and ⁻ may be important intermediates of the biosynthetic pathways of ⁻ and . Compounds and showed more potent inhibitory activity against -glucosidase compared with the positive control drug acarbose with IC value of 0.75 ± 0.018 mM and 1.64 ± 0.022 mM, respectively.
已被用作降血糖的民族医学药物。为了阐明有助于降血糖的生物活性化学成分，我们采用色谱技术对灵芝的子实体进行了化学研究，分离得到了 14 种化合物。通过广泛的光谱分析（质谱（MS）、核磁共振（NMR）、红外（IR）和紫外（UV））和与文献数据的比较，确定了它们的结构为三萜内酯（⁻和）和灵芝酸（⁻和 ⁻）。对化合物 ⁻、 ⁻、 ⁻和 ⁻进行了 -葡萄糖苷酶抑制活性评价。化合物 ⁻是新化合物。化合物 ⁻和 具有氧杂螺内酯部分的特征，由五元醚环、五元内酯环和特征 C-23 螺碳原子组成。在天然产物中，如此氧杂螺内酯部分存在于羊毛甾烷型三萜中是罕见的。化合物 ⁻和 ⁻可能是 ⁻和 的生物合成途径中的重要中间体。化合物 和 对 -葡萄糖苷酶的抑制活性强于阳性对照药物阿卡波糖，IC 值分别为 0.75 ± 0.018 mM 和 1.64 ± 0.022 mM。
