基于 NMR 的食用蘑菇中三萜类化合物的结构分类、鉴定和定量分析
NMR-based Structural Classification, Identification, and Quantification of Triterpenoids from Edible Mushroom .
机构信息
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
出版信息
J Agric Food Chem. 2020 Mar 4;68(9):2816-2825. doi: 10.1021/acs.jafc.9b07791. Epub 2020 Feb 20.
mushrooms have been widely used as functional food in China, Japan, and Korea. triterpenoids are deemed to be the main functional constituents. The structures of triterpenoids are complex but quite similar, which makes their analyses markedly limited. In this study, we developed a general 2D NMR method to differentiate triterpenoids, which classifies them into six types (A-F). Then, by the NMR-based isolation of A-F type triterpenoids from the fruiting bodies of , four new compounds (-) and eight known compounds (-) were obtained. Moreover, combined with spiking experiments in 1D and 2D NMR spectra, compounds , , and , which belong to triterpenoids of A and B types, were identified. At the end, to achieve a more extensive application for this NMR method, a qNMR method for the absolute quantification of , , and in the gross triterpenoids from was set up. The results showed that this NMR method is reliable for the NMR-guided isolation and quantification of triterpenoids in .
在中国、日本和韩国，蘑菇一直被广泛用作功能性食品。三萜类化合物被认为是主要的功能性成分。三萜类化合物的结构复杂但非常相似，这使得它们的分析受到明显限制。在这项研究中，我们开发了一种通用的二维 NMR 方法来区分三萜类化合物，将其分为六类（A-F）。然后，通过基于 NMR 的从 的子实体中分离 A-F 型三萜类化合物，从 中获得了四个新化合物（-）和八个已知化合物（-）。此外，通过在 1D 和 2D NMR 谱中的加标实验，鉴定了属于 A 型和 B 型三萜类化合物的化合物 - 。最后，为了更广泛地应用这种 NMR 方法，建立了一种 qNMR 方法，用于绝对定量 中的总三萜类化合物中的 、 和 。结果表明，该 NMR 方法可靠，可用于基于 NMR 指导的 中三萜类化合物的分离和定量。
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