正常及转化哺乳动物细胞的电特性。
Electrical properties of normal and transformed mammalian cells.
作者信息
Killion J J
出版信息
Biophys J. 1984 Mar;45(3):523-8. doi: 10.1016/S0006-3495(84)84189-2.
Abstract
The transmembrane potential difference, Em, and DC membrane resistance were measured in 3T3 and polyoma virus-transformed 3T3 cells. Em was a function of cell density and was -12 and -25 mV for the normal and transformed cells, respectively. The external concentrations of K+, Na+, and Cl were varied in order to study the nature of the differences between the two cell types. The relative permeability of ions was calculated to be: PNa/PK, 1.0; PCl/PK, 1.88; PNa/PCl, 0.53 for 3T3 cells, and 0.27, 1.75, and 0.15 for the transformed cells. In contrast to the normal cells, PNa/PK varied as a function of the external K+ concentration for the transformed cells. It was emphasized that the manipulation of variables directly affecting the electrical properties of cells also involves the indirect manipulation of a network of interconnected physiological determinants.
摘要
在3T3细胞和多瘤病毒转化的3T3细胞中测量跨膜电位差（Em）和直流膜电阻。Em是细胞密度的函数，正常细胞和转化细胞的Em分别为-12 mV和-25 mV。改变K⁺、Na⁺和Cl的细胞外浓度，以研究两种细胞类型之间差异的性质。计算出离子的相对渗透率为：3T3细胞的PNa/PK为1.0；PCl/PK为1.88；PNa/PCl为0.53，转化细胞的分别为0.27、1.75和0.15。与正常细胞不同，转化细胞的PNa/PK随细胞外K⁺浓度而变化。需要强调的是，直接影响细胞电学性质的变量操作也涉及对相互关联的生理决定因素网络的间接操作。
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