Kiefer H, Blume A J, Kaback H R
Proc Natl Acad Sci U S A. 1980 Apr;77(4):2200-4. doi: 10.1073/pnas.77.4.2200.
By monitoring differences in accumulation of the lipophilic cation [(3)H]tetraphenylphosphonium in media containing low or high potassium concentrations [Lichtshtein, D., Kaback, H. R. & Blume, A. J. (1979) Proc. Natl. Acad. Sci. USA 76, 650-654], the membrane potential of lymphocytes from various sources has been estimated. On the basis of this method, the potential of normal mouse spleen lymphocytes (T and B cells) is -65 +/- 2 mV (mean +/- SEM, interior negative). During the course of mitogenic stimulation by concanavalin A, lipopolysaccharide, or fetal calf serum, the membrane potential of murine spleen lymphocytes changes systematically according to the following pattern: (i) early depolarization lasting 2-3 hr, (ii) repolarization over the next 7 hr, and (iii) a final hyperpolarization phase during the last 24-48 hr. During repolarization and hyperpolarization, moreover, there is a direct correlation between the membrane potential and DNA synthesis, as judged by [(3)H]thymidine incorporation. By using isolated T and B cells, it is observed that concanavalin A depolarizes T cells only, whereas lipopolysaccharide depolarizes B cells only. Thus, both mitogens exhibit the same specificity for depolarization as for mitogenic stimulation. On the basis of these observations, it is suggested that the transition of lymphocytes from a resting state to mitotic activity is initiated by depolarization of the plasma membrane.
通过监测亲脂性阳离子[（3）H]四苯基鏻在低钾或高钾浓度培养基中的积累差异[利希施泰因，D.，卡巴克，H.R.和布卢姆，A.J.（1979年）《美国国家科学院院刊》76，650 - 654]，已估算出来自各种来源的淋巴细胞的膜电位。基于此方法，正常小鼠脾淋巴细胞（T细胞和B细胞）的电位为 - 65±2 mV（平均值±标准误，细胞内为负）。在伴刀豆球蛋白A、脂多糖或胎牛血清的促有丝分裂刺激过程中，小鼠脾淋巴细胞的膜电位按照以下模式系统性变化：（i）早期去极化持续2 - 3小时，（ii）在接下来的7小时内复极化，以及（iii）在最后24 - 48小时内的最终超极化阶段。此外，在复极化和超极化过程中，根据[（3）H]胸苷掺入判断，膜电位与DNA合成之间存在直接相关性。通过使用分离的T细胞和B细胞，观察到伴刀豆球蛋白A仅使T细胞去极化，而脂多糖仅使B细胞去极化。因此，两种促有丝分裂原在去极化方面表现出与促有丝分裂刺激相同的特异性。基于这些观察结果，有人提出淋巴细胞从静止状态向有丝分裂活性的转变是由质膜去极化引发的。
