Washetine Kevin, Kara-Borni Mehdi, Heeke Simon, Bonnetaud Christelle, Félix Jean-Marc, Ribeyre Lydia, Bence Coraline, Ilié Marius, Bordone Olivier, Pedro Marine, Maitre Priscilla, Tanga Virginie, Gormally Emmanuelle, Mossuz Pascal, Lorimier Philippe, Marquette Charles Hugo, Mouroux Jérôme, Cohen Charlotte, Lassalle Sandra, Long-Mira Elodie, Clément Bruno, Dagher Georges, Hofman Véronique, Hofman Paul
Hospital-Integrated Biobank (BB-0033-00025), Université Côte d'Azur, CHU de Nice, 06001 Nice CEDEX 1, France.
Team 4, Institute of Research on Cancer and Aging of Nice (IRCAN), Inserm U1081, CNRS UMR7284, Université Côte d'Azur, CHU de Nice, 06107 Nice CEDEX 2, France.
Cancers (Basel). 2018 Jun 11;10(6):195. doi: 10.3390/cancers10060195.
Collected specimens for research purposes may or may not be made available depending on their scarcity and/or on the project needs. Their protection against degradation or in the event of an incident is pivotal. Duplication and storage on a different site is the best way to assure their sustainability. The conservation of samples at room temperature (RT) by duplication can facilitate their protection. We describe a security system for the collection of non-small cell lung cancers (NSCLC) stored in the biobank of the Nice Hospital Center, France, by duplication and conservation of lyophilized (dried), encapsulated DNA kept at RT. Therefore, three frozen tissue collections from non-smoking, early stage and sarcomatoid carcinoma NSCLC patients were selected for this study. DNA was extracted, lyophilized and encapsulated at RT under anoxic conditions using the DNAshell technology. In total, 1974 samples from 987 patients were encapsulated. Six and two capsules from each sample were stored in the biobanks of the Nice and Grenoble (France) Hospitals, respectively. In conclusion, DNA maintained at RT allows for the conservation, duplication and durability of collections of interest stored in biobanks. This is a low-cost and safe technology that requires a limited amount of space and has a low environmental impact.
出于研究目的收集的标本可能会根据其稀缺性和/或项目需求而提供或不提供。保护它们不降解或在发生事故时不受影响至关重要。在不同地点进行复制和存储是确保其可持续性的最佳方法。通过复制在室温（RT）下保存样本可以促进对它们的保护。我们描述了一种安全系统，用于通过复制和保存保存在法国尼斯医院中心生物库中的非小细胞肺癌（NSCLC）冻干（干燥）、封装的DNA，并在室温下进行保存。因此，本研究选择了来自非吸烟、早期和肉瘤样癌NSCLC患者的三个冷冻组织样本。使用DNAshell技术在缺氧条件下于室温提取、冻干并封装DNA。总共对来自987名患者的1974个样本进行了封装。每个样本的六个和两个胶囊分别保存在法国尼斯和格勒诺布尔医院的生物库中。总之，在室温下保存的DNA能够实现保存在生物库中的感兴趣样本的保存、复制和耐久性。这是一种低成本且安全的技术，所需空间有限，对环境影响较小。
