School of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou, 225009, China.
Suzhou Customs (formerly Suzhou Entry-Exit Inspection and Quarantine Bureau), Suzhou, 215000, China.
Sci Rep. 2018 Jun 11;8(1):8850. doi: 10.1038/s41598-018-27213-7.
Liriomyza chinensis is a serious pest of onions in many countries, especially in East Asia. We sequenced the complete mitochondrial genome of this species and compared it with five other Agromyzidae species. The L. chinensis mitogenome is a double-stranded 16,175 bp circular molecule with an A + T content of 78.3%. It contains 37 genes and a control region as do the sequenced Liriomyza species. The mitogenomes of L. chinensis and other Agromyzidae species showed a clear bias in nucleotide composition with a positive AT-skew. Most PCGs used standard ATN as start codons, and TAN as termination codons. The tRNAs exhibited the typical clover-leaf structure, except for tRNASer and the two rRNA genes are conserved with those of other Agromyzids. The L. chinensis mitogenome control region included several conserved regions, including a poly-T, two (TA)n and one poly-A stretch, which are considered important replication and transcription. The 13 PCGs were used to study the phylogeny of L. chinensis and five related Agromyzids. Analysis by maximum likelihood, Bayesian inference and genetic distance suggest congruent phylogenetic relationships in Liriomyza spp. and provide a useful supplement to taxonomic classification by morphology.
南美斑潜蝇是许多国家，尤其是东亚国家洋葱的严重害虫。我们对该物种的完整线粒体基因组进行了测序，并与其他 5 种潜蝇科物种进行了比较。L. chinensis 线粒体基因组是一个双链 16175bp 环状分子，A+T 含量为 78.3%。它包含 37 个基因和一个控制区，与已测序的 Liriomyza 物种相同。L. chinensis 和其他潜蝇科物种的线粒体基因组在核苷酸组成上表现出明显的偏向性，具有正的 AT 倾斜。大多数 PCGs 使用标准的 ATN 作为起始密码子，TAN 作为终止密码子。tRNAs 表现出典型的三叶草结构，但 tRNASer 除外，并且两个 rRNA 基因与其他潜蝇科的基因保守。L. chinensis 线粒体基因组控制区包括几个保守区域，包括一个多-T、两个（TA）n 和一个多-A 延伸，这些区域被认为是重要的复制和转录区域。使用 13 个 PCGs 研究了 L. chinensis 和 5 种相关潜蝇科的系统发育。最大似然法、贝叶斯推断和遗传距离分析表明，Liriomyza spp. 中的系统发育关系一致，并为形态学分类提供了有用的补充。
