深共熔溶剂在从芽中提取芦丁及其体外抗氧化活性方面的应用
The application of deep eutectic solvent on the extraction and in vitro antioxidant activity of rutin from bud.
作者信息
Peng Fei, Xu Pei, Zhao Bing-Yi, Zong Min-Hua, Lou Wen-Yong
机构信息
1Laboratory of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640 China.
2Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou, 510640 China.
出版信息
J Food Sci Technol. 2018 Jun;55(6):2326-2333. doi: 10.1007/s13197-018-3151-9. Epub 2018 Apr 27.
Abstract
The extraction conditions and antioxidant activities of rutin from bud by deep eutectic solvents were investigated. Box-Behnken design was used to optimize the extraction conditions and the scavenging activities of DPPH, O and ·OH of purified rutin were evaluated. The highest yield of 279.8 mg/g was achieved in the extraction medium of choline chloride/triethlene glycol (1/4) under the optimum conditions: water content of the DES 18.1%, extraction time 28.3 min, extraction temperature 70 °C and liquid-solid ratio 10 mg/1 g. The highest extraction amount was slightly different from the predicted value of the established second-order polynomial equation. In addition, The EC of DPPH scavenging, O scavenging and ·OH scavenging of rutin were 5.68 µg/mL, 0.19 and 0.28 mg/mL, respectively. The above results indicate rutin extracted by the choline chloride/triethylene glycol has excellent antioxidant activity and was an admirable free radical scavenger.
摘要
研究了采用深共熔溶剂从芽中提取芦丁的条件及其抗氧化活性。采用Box-Behnken设计优化提取条件，并评估了纯化芦丁对DPPH、O和·OH的清除活性。在最佳条件下：深共熔溶剂含水量18.1%、提取时间28.3分钟、提取温度70℃、液固比10mg/1g，在氯化胆碱/三甘醇（1/4）提取介质中获得了最高产量279.8mg/g。最高提取量与所建立的二阶多项式方程的预测值略有不同。此外，芦丁对DPPH清除、O清除和·OH清除的EC分别为5.68μg/mL、0.19和0.28mg/mL。上述结果表明，通过氯化胆碱/三甘醇提取的芦丁具有优异的抗氧化活性，是一种令人满意的自由基清除剂
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