Chu JianZhi, Ming YongFei, Cui Qi, Zheng Na, Yang ShuDe, Li WeiHuan, Gao Hongwei, Zhang Rui, Cheng XianHao
Shandong Key Lab of Edible Mushroom Technology, School of Agriculture, Ludong University, Yantai, 264025, China.
School of Life Science, Ludong University, Yantai, 264025, Shandong, China.
BMC Biotechnol. 2022 Mar 7;22(1):9. doi: 10.1186/s12896-022-00739-5.
Antrodia cinnamomea, a rare medicinal fungus, has been increasingly studied in recent years because of its abundant secondary metabolites which are beneficial to humans. However, there is a lack of research on its polyphenols which are of good research value due to their antioxidant, anti-inflammatory, hypoglycemic and other activities.
In this study, the effects of different extraction conditions on the yield of its polyphenols were investigated. Deep-Eutectic Solvents composed of choline chloride and malonic acid had the best extraction efficiency, with the optimal extraction conditions being as follows: a solid-liquid ratio of 40 mg/mL, an extraction temperature of 55 °C, an extraction time of 70 min and a DES with 20% water content. Under these conditions, the extraction yield of polyphenols reached 22.09 mg/g which was about 2 times that of alcohol-based extraction (10.95 mg/g). In vitro antioxidant test results further showed that polyphenols from A. cinnamomea had strong antioxidant activities. When the concentration of polyphenols reached 0.1 mg/mL of polyphenols, the scavenging activity of free radical basically reached its maximum, with values of 94.10%, 83.34% and 95.42% for DPPH, ABTS and ·OH scavenging. In this case, the corresponding IC values were 0.01, 0.014 and 0.007 mg/mL, respectively.
This study lays the foundation for the efficient extraction and application of polyphenols from A. cinnamomea.
樟芝是一种珍稀药用真菌，近年来因其富含对人体有益的次生代谢产物而受到越来越多的研究。然而，对其多酚类物质的研究较少，由于其具有抗氧化、抗炎、降血糖等活性，具有良好的研究价值。
本研究考察了不同提取条件对其多酚产量的影响。由氯化胆碱和丙二酸组成的深共熔溶剂提取效率最佳，最佳提取条件如下：固液比40 mg/mL，提取温度55℃，提取时间70 min，含水量20%的深共熔溶剂。在此条件下，多酚的提取率达到22.09 mg/g，约为醇提（10.95 mg/g）的2倍。体外抗氧化试验结果进一步表明，樟芝多酚具有较强的抗氧化活性。当多酚浓度达到0.1 mg/mL时，自由基清除活性基本达到最大值，DPPH、ABTS和·OH清除率分别为94.10%、83.34%和95.42%。此时，相应的IC值分别为0.01、0.014和0.007 mg/mL。
本研究为樟芝多酚的高效提取和应用奠定了基础。
