Bauer H J, Monreal G
J Virol Methods. 1985 May;11(1):87-92. doi: 10.1016/0166-0934(85)90127-2.
Avian adeno-associated virus (AAAV) pre-purified by Uvasol extraction, one discontinuous and two CsCl equilibrium density gradients, was still considerably contaminated with avian adenovirus (CELO strain). Four different approaches were investigated in attempts to improve the elimination of the contaminating CELO virus. The contaminations were assayed by double immunodiffusion and indirect immunofluorescence. The immunoprecipitation of CELO virus with antiserum and protein A-Sepharose is the most effective method of obtaining purified AAAV free of CELO virus.
通过Uvasol萃取、一个不连续和两个CsCl平衡密度梯度预纯化的禽腺相关病毒（AAAV），仍然被禽腺病毒（CELO株）大量污染。研究了四种不同方法，试图改进对污染的CELO病毒的去除。通过双向免疫扩散和间接免疫荧光检测污染情况。用抗血清和蛋白A-琼脂糖对CELO病毒进行免疫沉淀，是获得不含CELO病毒的纯化AAAV的最有效方法。
