Sakaoka H, Aomori T, Honda O, Saheki Y, Ishida S, Yamanishi S, Fujinaga K
J Infect Dis. 1985 Jul;152(1):190-7. doi: 10.1093/infdis/152.1.190.
An attempt was made to classify herpes simplex virus type 1 (HSV-1) isolates into subtypes on the basis of the combination of the gain or loss of specific cleavage sites of HSV-1 genomes with each of three restriction endonucleases (Bam HI, Kpn I, and Sal I). According to the criteria we used for the determination of HSV-1 subtypes, 93 strains of HSV-1 that were isolated in three areas of Japan (Sapporo, Tottori, and Kagawa) were tentatively classified into eight subtypes: subtypes A-H. The bulk of the strains (84 of 93) fell into three subtypes: A, C, and H. There were highly significant differences (P less than .01) in the proportion of subtypes A and H that were isolated in Sapporo as compared with those isolated in Tottori and in Kagawa, which are geographically far from Sapporo. No significant differences, however, were found in subtypes isolated in Tottori as compared with those isolated in Kagawa, which are geographically close to each other. These data suggest that there might be a correlation between the genome structure of HSV-1 and the areas of their isolation in Japan.
人们尝试根据1型单纯疱疹病毒（HSV - 1）基因组与三种限制性内切酶（Bam HI、Kpn I和Sal I）各自的特定切割位点的增减组合，将HSV - 1分离株分为不同亚型。根据我们用于确定HSV - 1亚型的标准，在日本三个地区（札幌、鸟取和香川）分离出的93株HSV - 1被初步分为八个亚型：A - H亚型。大部分菌株（93株中的84株）属于三个亚型：A、C和H。与在距离札幌较远的鸟取和香川分离出的菌株相比，在札幌分离出的A和H亚型的比例存在极显著差异（P小于0.01）。然而，在地理位置彼此接近的鸟取和香川分离出的亚型之间未发现显著差异。这些数据表明，HSV - 1的基因组结构与其在日本的分离地区之间可能存在相关性。
