Hasanvand Davood, Amiri Iraj, Soleimani Asl Sara, Saidijam Massoud, Shabab Nooshin, Artimani Tayebe
a Anatomy Department, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
b Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Can J Physiol Pharmacol. 2018 Sep;96(9):963-969. doi: 10.1139/cjpp-2017-0784. Epub 2018 Jun 12.
CeO nanoparticles (CNPs) as effective ROS scavengers exhibit potent antioxidant activity. In this study the effect of CNPs investigated was on HO-1, NQO1, and GCLC expression in the streptozotocin (STZ)-induced diabetic rats. Twenty-four male Wistar rats were divided into 4 groups: controls did not receive any treatment; diabetic rats received STZ (60 mg/kg daily); CNPs group received CNPs 30 mg/kg daily for 2 weeks; and rats in STZ + CNPs group received CNPs 30 mg/kg daily for 2 weeks following STZ injection. Oxidative stress was evaluated by measurement of total antioxidant capacity (TAC) and total oxidative status (TOS levels). HO-1, NQO1, and GCLC expression was measured using quantitative real-time PCR. Following STZ injection, significant lower levels of TAC and higher levels of TOS were observed. CNPs could alleviate deleterious effects of diabetes through the enhancement of TAC levels and a significant decline in TOS levels. HO-1, NQO1, and GCLC expression in the diabetic rats were lower than controls. HO-1, NQO1, and GCLC was upregulated in the diabetic rats treated with CNPs. There were significant correlations between NQO1 and GCLC, NQO1 and HO-1, and between HO-1 and GCLC expression. Moreover, Nrf2 was associated with NQO1, GCLC, and HO-1 expression. CNPs as Nrf2 upregulator confer protection against oxidative stress in the testes of STZ-induced diabetic rats by upregulating HO-1, GCLC, and NQO1 cytoprotective genes.
二氧化铈纳米颗粒（CNPs）作为有效的活性氧清除剂，具有强大的抗氧化活性。在本研究中，所研究的CNPs对链脲佐菌素（STZ）诱导的糖尿病大鼠中血红素加氧酶-1（HO-1）、醌氧化还原酶1（NQO1）和谷氨酸半胱氨酸连接酶催化亚基（GCLC）的表达产生影响。将24只雄性Wistar大鼠分为4组：对照组不接受任何治疗；糖尿病大鼠接受STZ（每日60 mg/kg）；CNPs组每日接受30 mg/kg的CNPs，持续2周；STZ + CNPs组的大鼠在注射STZ后每日接受30 mg/kg的CNPs，持续2周。通过测量总抗氧化能力（TAC）和总氧化状态（TOS水平）来评估氧化应激。使用定量实时聚合酶链反应测量HO-1、NQO1和GCLC的表达。注射STZ后，观察到TAC水平显著降低，TOS水平升高。CNPs可通过提高TAC水平和显著降低TOS水平来减轻糖尿病的有害影响。糖尿病大鼠中HO-1、NQO1和GCLC的表达低于对照组。在用CNPs治疗的糖尿病大鼠中，HO-1、NQO1和GCLC上调。NQO1与GCLC、NQO1与HO-1以及HO-1与GCLC表达之间存在显著相关性。此外，核因子E2相关因子2（Nrf2）与NQO1、GCLC和HO-1表达相关。作为Nrf2上调剂的CNPs通过上调HO-1、GCLC和NQO1细胞保护基因，对STZ诱导的糖尿病大鼠睾丸中的氧化应激提供保护。
