一种用于检测自来水中和活细胞中亚氯酸的高选择性荧光探针。
A highly selective fluorescent probe for the detection of hypochlorous acid in tap water and living cells.
机构信息
Henan Joint International Research Laboratory of Environmental Pollution Control Materials, College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, China.
Henan Joint International Research Laboratory of Environmental Pollution Control Materials, College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Oct 5;203:415-420. doi: 10.1016/j.saa.2018.06.012. Epub 2018 Jun 3.
A turn-on fluorescent probe (DAME) for sensing hypochlorous acid (HClO) with excellent selectivity was presented. The fluorescent probe was composed of coumarin derivative as the fluorophore and dimethylcarbamothioic chloride group with a sulfide moiety as modulator. Additionally, the sulfide moiety would be oxidized by HClO, and then free dye of coumarin derivate was released and exhibited significant fluorescence. In addition, the probe could respond to HClO in solutions within 60 s and the limit of detection was down to 34.75 nM. Moreover, the probe was used for the detection of HClO in tap water through the home-made test paper. And confocal images confirmed that probe DAME could be used for recognizing HClO in living cells.
呈现了一种用于检测次氯酸（HClO）的荧光探针（DAME），其具有出色的选择性。该荧光探针由香豆素衍生物作为荧光团和二甲氨基甲硫酰氯基团与含硫部分作为调节剂组成。此外，HClO 会氧化含硫部分，然后释放出香豆素衍生物的游离染料，并表现出显著的荧光。此外，探针可以在 60 秒内对溶液中的 HClO 做出响应，检测限低至 34.75 nM。此外，该探针通过自制试纸用于检测自来水中的 HClO。共聚焦图像证实探针 DAME 可用于识别活细胞中的 HClO。
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