使用生物反应器培养人视网膜类器官可提高光感受器产量。
Use of bioreactors for culturing human retinal organoids improves photoreceptor yields.
机构信息
Department of Genetics, UCL Institute of Ophthalmology, 11-43 Bath Street, London, EC1V 9EL, UK.
NIHR Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology, City Road, London, EC1V 2PD, UK.
出版信息
Stem Cell Res Ther. 2018 Jun 13;9(1):156. doi: 10.1186/s13287-018-0907-0.
BACKGROUND
The use of human pluripotent stem cell-derived retinal cells for cell therapy strategies and disease modelling relies on the ability to obtain healthy and organised retinal tissue in sufficient quantities. Generating such tissue is a lengthy process, often taking over 6 months of cell culture, and current approaches do not always generate large quantities of the major retinal cell types required.
METHODS
We adapted our previously described differentiation protocol to investigate the use of stirred-tank bioreactors. We used immunohistochemistry, flow cytometry and electron microscopy to characterise retinal organoids grown in standard and bioreactor culture conditions.
RESULTS
Our analysis revealed that the use of bioreactors results in improved laminar stratification as well as an increase in the yield of photoreceptor cells bearing cilia and nascent outer-segment-like structures.
CONCLUSIONS
Bioreactors represent a promising platform for scaling up the manufacture of retinal cells for use in disease modelling, drug screening and cell transplantation studies.
背景
将人多能干细胞衍生的视网膜细胞用于细胞治疗策略和疾病建模依赖于能够以足够的数量获得健康且有序的视网膜组织。生成这种组织是一个漫长的过程，通常需要超过 6 个月的细胞培养，并且当前的方法并不总是能够生成大量所需的主要视网膜细胞类型。
方法
我们改编了之前描述的分化方案，以研究搅拌罐生物反应器的使用。我们使用免疫组织化学、流式细胞术和电子显微镜来描述在标准和生物反应器培养条件下生长的视网膜类器官。
结果
我们的分析表明，使用生物反应器可改善层状分层，并增加具有纤毛和新生外节样结构的感光细胞的产量。
结论
生物反应器为扩大用于疾病建模、药物筛选和细胞移植研究的视网膜细胞的制造提供了一个有前途的平台。
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