College of Pharmacy, Jinan University, Guangzhou, China.
Shenzhen Traditional Chinese Medicine Hospital, Shenzhen, China.
J Microencapsul. 2020 Sep;37(6):403-412. doi: 10.1080/02652048.2020.1767224. Epub 2020 May 26.
In this study, we prepared spirulina polysaccharides into spirulina polysaccharide-loaded nanoemulsions (SPS-NEs), and determined the antitumor effect of SPS-NEs, when combined with paclitaxel (PTX). SPS-NEs were prepared by a phase transformation method. The Characterisation and stability of SPS-NEs was measured. The antitumor effect of SPS-NEs combined with PTX was determined by S180 cells or RAW 264.7 macrophages and S180 tumour-bearing mice. SPS-NEs were spherical and stable, the particle size of SPS-NEs was 84.6 ± 3.31 nm, PDI = 0.235 ± 0.02. PTX + SPS-NEs exhibited a much greater toxicity against RAW 264.7 cells than PTX. PTX + SPS-NEs increased the release of NO, IL-6 and TNF-α, and the expression of p-p65 NF-κB, p-I-κB, TLR4. In addition, PTX + SPS-NEs significantly inhibited tumour growth by 72.82% and increased the secretion of serum IL-2, TNF-α and IFN-γ. SPS-NEs can regulate immunity through TLR4/NF-κB signalling pathways, which enhances the anti-tumour effect of PTX.
在这项研究中，我们制备了螺旋藻多糖负载纳米乳（SPS-NE），并确定了 SPS-NE 与紫杉醇（PTX）联合使用时的抗肿瘤作用。SPS-NE 通过相转变法制备。测量了 SPS-NE 的特性和稳定性。通过 S180 细胞或 RAW 264.7 巨噬细胞和 S180 荷瘤小鼠来确定 SPS-NE 与 PTX 联合的抗肿瘤作用。SPS-NE 呈球形且稳定，SPS-NE 的粒径为 84.6±3.31nm，PDI=0.235±0.02。与 PTX 相比，PTX+SPS-NE 对 RAW 264.7 细胞的毒性更大。PTX+SPS-NE 增加了 NO、IL-6 和 TNF-α的释放，以及 p-p65 NF-κB、p-I-κB、TLR4 的表达。此外，PTX+SPS-NE 通过 72.82%显著抑制肿瘤生长，并增加血清 IL-2、TNF-α 和 IFN-γ的分泌。SPS-NE 可以通过 TLR4/NF-κB 信号通路调节免疫，从而增强 PTX 的抗肿瘤作用。
