Mihály A, Tóth G, Szente M, Joó F
Acta Neuropathol. 1985;66(2):145-54. doi: 10.1007/BF00688690.
Focal seizure was induced in rat and cat neocortex by the topical application of aminopyridines. The epileptic character of the focal events was followed by surface and intracellular recordings of seizure activity. The pathologic alterations in the neurons, the glial cells, and the protein permeability of the neocortical blood vessels were investigated by means of light microscopy, using standard histological stainings and immunohistochemical detection of endogenous serum albumin. Diffusion of [3H] 4-aminopyridine in the neocortex was studied by light-microscopic autoradiography. The spreading of the neuropathologic changes strictly followed the diffusion of the tritiated compound, suggesting the gradual involvement of neocortical layers in the seizure process.
通过局部应用氨基吡啶在大鼠和猫的新皮质中诱发局灶性癫痫发作。通过表面和细胞内记录癫痫发作活动来追踪局灶性事件的癫痫特征。使用标准组织学染色和内源性血清白蛋白的免疫组织化学检测，通过光学显微镜研究神经元、神经胶质细胞的病理改变以及新皮质血管的蛋白质通透性。通过光学显微镜放射自显影研究[3H] 4-氨基吡啶在新皮质中的扩散。神经病理变化的扩散严格遵循氚标记化合物的扩散，表明新皮质层逐渐参与癫痫发作过程。
