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两种用于粪便中人类轮状病毒快速检测的商业试剂盒评估:乳胶凝集试验Rotalex和轮状病毒酶联免疫吸附测定试剂盒。

Evaluation of two commercial kits for rapid detection of human rotavirus in feces: Rotalex, a latex agglutination test and Rotavirus ELISA Kit.

作者信息

Jensen A

出版信息

Acta Pathol Microbiol Immunol Scand B. 1985 Apr;93(2):159-60. doi: 10.1111/j.1699-0463.1985.tb02869.x.

Abstract

96 fecal specimens from children with symptoms of gastroenteritis were tested with a latex agglutination test (Rotalex, Orion Diagnostica, Finland) and an enzyme-linked immunosorbent test (Rotavirus ELISA Kit, Dakopatts, Copenhagen, Denmark). Using the ELISA method as reference, the latex agglutination (LX) came out with three non-specific and two false negative results. The LX test was found to be a rapid, practical and non-expensive choice for a laboratory examinating few samples daily.

摘要

采用乳胶凝集试验(芬兰奥立安诊断公司的Rotalex)和酶联免疫吸附试验(丹麦哥本哈根达科帕茨公司的轮状病毒ELISA试剂盒)对96份有肠胃炎症状儿童的粪便样本进行检测。以ELISA方法作为参照,乳胶凝集试验出现了3例假阳性和2例假阴性结果。对于每天检测样本较少的实验室而言,乳胶凝集试验是一种快速、实用且成本较低的选择。

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