Grauballe P C, Jarzabek Z
Acta Virol. 1984 Jan;28(1):59-63.
94 faecal samples from infants and children suffering of acute gastroenteritis were investigated for rotavirus by indirect double antibody sandwich ELISA kit (WHO, Geneva), Rotavirus ELISA kit (DAKOPATTS A/S, Copenhagen) and Rotalex latex-agglutination kit (Orion Diagnostica, Helsinki). The ELISA techniques gave almost identical results and seemed to be of same sensitivity and specificity. Rotalex agglutination had an overall agreement of 88% with ELISA. It is concluded that strongly positive reactions found by Rotalex may be regarded as true positive reactions, whereas samples producing weakly positive and/or negative reactions should be retested in a more specific and sensitive assay, such as enzyme linked immunosorbent-assay (ELISA).
采用间接双抗体夹心酶联免疫吸附测定试剂盒（世界卫生组织，日内瓦）、轮状病毒酶联免疫吸附测定试剂盒（达科帕茨公司，哥本哈根）和罗塔莱克斯乳胶凝集试剂盒（奥立安诊断公司，赫尔辛基），对94份患有急性肠胃炎的婴幼儿粪便样本进行了轮状病毒检测。酶联免疫吸附测定技术得出的结果几乎相同，且似乎具有相同的灵敏度和特异性。罗塔莱克斯凝集法与酶联免疫吸附测定法的总体一致性为88%。得出的结论是，罗塔莱克斯检测出的强阳性反应可被视为真阳性反应，而产生弱阳性和/或阴性反应的样本应采用更具特异性和灵敏度的检测方法（如酶联免疫吸附测定法）重新检测。
