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[使用商业免疫酶和乳胶制剂检测人轮状病毒抗原]

[The use of commercial immunoenzyme and latex preparations for the demonstration of human rotavirus antigens].

作者信息

Gorbachev E N, Verbov V N, Artiukhov A I, Noskov F S

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1989 Aug(8):68-73.

PMID:2555984
Abstract

The results of using enzyme immunoassay and latex preparations for the diagnosis of rotavirus gastroenteritis are presented. High effectiveness of the enzyme immunoassay system developed in the USSR with latex diagnostic agents, such as Rotalex (Orion Diagnostica, Finland), Slidex Rota Kit (BioMérieux, France), The Wellcome Rotavirus Latex Kit (Wellcome Foundation Ltd., Great Britain), 48-63% and 21-41% respectively, has been noted. The results of the comparison of the system developed in the USSR with Wellcozyme Rotavirus, an enzyme immunoassay system manufactured by Wellcome Foundation Ltd. (Great Britain), are practically comparable. The results of the block test and the confirmation test used for control indicate that the Soviet preparation is specific. Materials on the practical evaluation of the assay system at health institutions are presented. Good prospects for the use of this system in the diagnosis of rotavirus gastroenteritis, as well as in the realization of epidemiological surveillance on this infection, are substantiated.

摘要

本文介绍了使用酶免疫测定法和乳胶制剂诊断轮状病毒肠胃炎的结果。已注意到苏联研发的酶免疫测定系统与乳胶诊断试剂(如芬兰奥立安诊断公司的Rotalex、法国生物梅里埃公司的Slidex Rota Kit、英国威康基金会有限公司的The Wellcome Rotavirus Latex Kit)配合使用时的高效性,分别为48 - 63%和21 - 41%。苏联研发的系统与英国威康基金会有限公司生产的酶免疫测定系统Wellcozyme Rotavirus的比较结果基本相当。用于对照的阻断试验和确认试验结果表明苏联的制剂具有特异性。文中还介绍了医疗机构对该测定系统进行实际评估的材料。证实了该系统在诊断轮状病毒肠胃炎以及对这种感染进行流行病学监测方面具有良好的应用前景。

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