Wang Fang, Wang Hongxia, Jin Danting, Zhang Yang
Department of Gynecology Department of Pathology Department of Clinical Laboratory, The First People's Hospital of Lianyungang, Xuzhou Medical University Affiliated Hospital of Lianyungang, Lianyungang, Jiangsu, China.
Medicine (Baltimore). 2018 Jun;97(24):e10853. doi: 10.1097/MD.0000000000010853.
Clinical studies have exhibited microRNAs or cytokines could be used as new biomarkers in the diagnosis of endometriosis, respectively. The purpose of this study was to investigate the role of serum miR-17, IL-4, and IL-6 as early diagnostic markers of endometriosis. One hundred forty patients aged 22 to 45 years were recruited, 80 patients with pathologically confirmed endometriosis were assigned to endometriosis group whereas the remaining 60 patients were in the control group. The blood samples were collected immediately before laparoscopy and analyzed using real-time quantitative PCR analysis. In patients with endometriosis, the level of miR-23b decreased significantly, the levels of IL-4 and IL-6 increased remarkably compared with that in patients without endometriosis. Correlation analysis revealed miR-17 levels were negatively correlated with IL-4 (r = -0.974, P < .05) and IL-6 (r = -0.944, P < .05). The ROC curve manifested joint of miR-17 and selected cytokines could improve the diagnostic power with an AUC of 0.84 (95% CI: 0.75-0.96). In short, the present study characterizes the role of miR-17, IL-4, and IL-6 in the pathogenesis of endometriosis, suggesting the feasibility of using miR-17 and selected cytokines as a noninvasive diagnostic test for the detection of endometriosis.
临床研究表明，微小RNA或细胞因子可分别作为子宫内膜异位症诊断的新生物标志物。本研究的目的是探讨血清miR-17、白细胞介素-4（IL-4）和白细胞介素-6（IL-6）作为子宫内膜异位症早期诊断标志物的作用。招募了140例年龄在22至45岁之间的患者，其中80例经病理证实为子宫内膜异位症的患者被分配到子宫内膜异位症组，其余60例患者为对照组。在腹腔镜检查前立即采集血样，并使用实时定量聚合酶链反应分析进行检测。与无子宫内膜异位症的患者相比，子宫内膜异位症患者中miR-23b水平显著降低，IL-4和IL-6水平显著升高。相关性分析显示，miR-17水平与IL-4（r = -0.974，P < 0.05）和IL-6（r = -0.944，P < 0.05）呈负相关。ROC曲线显示，miR-17与所选细胞因子联合使用可提高诊断效能，曲线下面积（AUC）为0.84（95%可信区间：0.75 - 0.96）。简而言之，本研究阐述了miR-17、IL-4和IL-6在子宫内膜异位症发病机制中的作用，表明使用miR-17和所选细胞因子作为检测子宫内膜异位症的非侵入性诊断试验具有可行性。
