Lin Chunli, Zeng Saili, Li Miaojie
Department of Gynecology, Hunan Province Maternal and Child Health Care Hospital, 53 Xiangchun Road, Kaifu District, Changsha, 410008, Hunan, China.
Department of Respiratory Medicine, The Second Hospital of University of South China, 30 Jiefang Road, Shigu District, Hengyang, 421000, Hunan, China.
Arch Gynecol Obstet. 2023 Jan;307(1):169-177. doi: 10.1007/s00404-022-06492-6. Epub 2022 Apr 3.
Endometriosis (EMT) is a chronic benign disease with high prevalence. This study investigated the diagnostic value of serum miR-17-5p, miR-424-5p, and their combined expressions for EMT.
Total 80 EMT patients of reproductive age who underwent laparoscopy or laparotomy and were confirmed by pathological examination were included as the study subjects, and another 80 healthy women of reproductive age receiving gynecological examination and ultrasonography with no pelvic abnormalities were selected as the control group. The whole blood samples of enrolled subjects were collected and clinical characteristics were recorded. The miR-17-5p, miR-424-5p, VEGFA, IL-4, and IL-6 levels in the serum were measured. ROC curve was used to evaluate the diagnostic efficacy of miR-17-5p and miR-424-5p expressions for EMT. Pearson correlation was performed to analyze the correlation of miR-17-5p and miR-424-5p with clinical indexes in EMT patients.
miR-17-5p and miR-424-5p were downregulated in EMT patients. For diagnosing EMT, the AUC of miR-17-5p was 0.865 and cutoff value was 0.890 (91.3% sensitivity and 85% specificity), the AUC of miR-424-5p was 0.737, and cutoff value was 0.915 (98.8% sensitivity and 61.2% specificity), and the AUC of miR-424-5p combined with miR-17-5p was 0.938 and cutoff value was 2.205 (93.8% sensitivity and 88.7% specificity), with the diagnostic efficacy higher than miR-424-5p or miR-17-5p alone. miR-17-5p and miR-424-5p expressions were negatively correlated with dysmenorrhea, infertility, pelvic pain, and rASRM stage, but not with age, BMI, menstrual disorder, and nulliparity. VEGFA, IL-4, IL-6, and CA-125 were increased in EMT patients and were inversely associated with miR-17-5p and miR-424-5p.
miR-424-5p combined with miR-17-5p has high diagnostic efficacy for EMT.
子宫内膜异位症（EMT）是一种高发性慢性良性疾病。本研究探讨血清miR-17-5p、miR-424-5p及其联合表达对EMT的诊断价值。
选取80例接受腹腔镜或剖腹手术且经病理检查确诊的育龄期EMT患者作为研究对象，另选取80例接受妇科检查及超声检查且无盆腔异常的育龄期健康女性作为对照组。收集入选对象的全血样本并记录临床特征。检测血清中miR-17-5p、miR-424-5p、血管内皮生长因子A（VEGFA）、白细胞介素-4（IL-4）和白细胞介素-6（IL-6）水平。采用ROC曲线评估miR-17-5p和miR-424-5p表达对EMT的诊断效能。采用Pearson相关性分析miR-17-5p和miR-424-5p与EMT患者临床指标的相关性。
EMT患者中miR-17-5p和miR-424-5p表达下调。对于EMT的诊断，miR-17-5p的曲线下面积（AUC）为0.865，临界值为0.890（灵敏度91.3%，特异度85%）；miR-424-5p的AUC为0.737，临界值为0.915（灵敏度98.8%，特异度61.2%）；miR-424-5p与miR-17-5p联合检测的AUC为0.938，临界值为2.205（灵敏度93.8%，特异度88.7%），其诊断效能高于单独检测miR-424-5p或miR-17-5p。miR-17-5p和miR-424-5p表达与痛经、不孕、盆腔疼痛及美国生殖医学学会（rASRM）分期呈负相关，但与年龄、体重指数（BMI）、月经紊乱及未生育无关。EMT患者中VEGFA、IL-4、IL-6和糖类抗原125（CA-125）升高，且与miR-17-5p和miR-424-5p呈负相关。
miR-424-5p与miR-17-5p联合检测对EMT具有较高的诊断效能。
