Komolkriengkrai Manaras, Nopparat Jongdee, Vongvatcharanon Uraporn, Anupunpisit Vipavee, Khimmaktong Wipapan
Department of Anatomy, Faculty of Science, Prince of Songkla University, Hatyai, Songkhla 90110, Thailand.
Department of Anatomy, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand.
Exp Ther Med. 2019 Aug;18(2):1164-1174. doi: 10.3892/etm.2019.7664. Epub 2019 Jun 11.
Abnormalities in insulin hormone levels leads to a hyperglycemic condition of diabetic mellitus. Hyperglycemia seriously induces organ and system destructions. The excessive accumulation of collagen fiber deposits occurs in inflammatory and reorganization processes of chronic liver diseases in type I insulin-dependent diabetes. Regarding the research objective, glabridin (GLB), an active compound of licorice, was used as a daily supplement (40 mg/kg) in order to decrease hepatocyte destruction and collagen deposition in liver tissue of diabetic animals induced by streptozotocin. A total of 40 were randomly allocated to five groups (each, n=10), control, control treated with GLB (GLB), diabetic rats (DM) injected with single dose of streptozotocin (60 mg/kg) to induce a diabetic condition, diabetic rats receiving GLB (DM+GLB; 40 mg/kg) and diabetic rats treated with glibenclamide (DM+GL; 4 mg/kg). Characteristic histopathological changes in liver cells and tissues of rats were determined by Masson's trichrome staining and transmission electron microscopy (TEM). Western blotting was used to detect the expression of the key markers, collagen type I and fibronectin proteins. The histological investigation of liver tissue of the DM group revealed that the collagen fiber deposition was increased in the periportal, pericentral and perisinusoidal spaces compared with controls. Hepatocytes appeared as small and fragmented cells in TEM examination. Collagenization of the perisinusoidal space was recently demonstrated to represent a new aspect of the microvascular abnormalities and liver fibrosis. Healthy hepatocytes with round nucleus were observed following supplementation of glabridin. In addition, collagen fiber deposition was reduced in the area adjacent to the perisinusoidal space. The expression of collagen type I and fibronectin decreased strongly following glabridin supplementation in DM+GLB rats compared with DM rats, indicating that the hepatic tissue reorganization regained its normal morphology. These findings suggest that it may be beneficial to examine the role of glabridin as a therapeutic agent in diabetes treatment in future research.
胰岛素激素水平异常会导致糖尿病的高血糖状况。高血糖会严重引发器官和系统损害。在I型胰岛素依赖型糖尿病的慢性肝病炎症和重塑过程中，会出现胶原纤维沉积物过度积累的情况。关于研究目标，光甘草定（GLB），一种甘草的活性化合物，被用作每日补充剂（40毫克/千克），以减少链脲佐菌素诱导的糖尿病动物肝脏组织中的肝细胞破坏和胶原沉积。总共40只动物被随机分为五组（每组n = 10），分别为对照组、接受GLB治疗的对照组（GLB组）、注射单剂量链脲佐菌素（60毫克/千克）以诱导糖尿病状态的糖尿病大鼠（DM组）、接受GLB的糖尿病大鼠（DM + GLB组；40毫克/千克）和接受格列本脲治疗的糖尿病大鼠（DM + GL组；4毫克/千克）。通过Masson三色染色和透射电子显微镜（TEM）确定大鼠肝细胞和组织的特征性组织病理学变化。使用蛋白质免疫印迹法检测关键标志物I型胶原蛋白和纤连蛋白的表达。DM组肝脏组织的组织学研究表明，与对照组相比，门静脉周围、中央静脉周围和肝血窦周围间隙中的胶原纤维沉积增加。在TEM检查中，肝细胞呈现为小而破碎的细胞。最近证明肝血窦周围间隙的胶原化代表了微血管异常和肝纤维化的一个新方面。补充光甘草定后观察到具有圆形细胞核的健康肝细胞。此外，肝血窦周围间隙相邻区域的胶原纤维沉积减少。与DM大鼠相比，DM + GLB大鼠补充光甘草定后I型胶原蛋白和纤连蛋白的表达强烈下降，表明肝组织重塑恢复了正常形态。这些发现表明，在未来的研究中研究光甘草定作为糖尿病治疗药物的作用可能是有益的。
