Yin Xu, Zhang Qian, Zhang He-Xin-Ge, Wuken Sha-Na, Li Jun-Jun, Jiao Shun-Gang, Yang Feng-Qing, Tu Peng-Fei, Chai Xing-Yun
Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.
School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China.
Zhongguo Zhong Yao Za Zhi. 2018 May;43(9):1758-1763. doi: 10.19540/j.cnki.cjcmm.20180115.014.
Nine alkaloids and two phenolic glycosides were isolated from EtOH extract of the whole plants of Corydalis hendersonii by various chromatographic techniques including silica gel, ODS, Sephadex LH-20, and semi-preparative HPLC. Their structures were identified as groenlandicine (1), berberine (2), protopine (3), cryptopine (4), N-trans-feruloyloctopamine(5), 3-(4-hydroxy-3-methoxyphenyl)-N-[2-(4-hydroxyphenyl)-2-methoxyethyl] acrylamide (6), N-cis-p-coumaroyloctopamine (7), N-trans-p-coumaroylnoradrenline (8),N-cis-feruloyloctopamine (9), apocynin (10), and glucoacetosyringone (11) by the spectroscopic data analysis and comparison with those in the literature. Among them, compounds 10 and 11 were isolated from this genus for the first time, and 1, 2, and 5-9 were isolated from the species for the first time. All isolates were tested for their protection for in vitro PC12 cell line and antiplatelet aggregation activity. The results showed that compounds 5 and 7 displayed protective effects at a concentration of 10 μmol·L⁻¹, and compound 2 showed antiplatelet aggregation activity induced by THR, ADP, and AA, and compound 3 exhibted inhibitory effect induced by THR.
通过硅胶、ODS、葡聚糖凝胶LH - 20和半制备高效液相色谱等多种色谱技术，从滇西紫堇全草的乙醇提取物中分离得到了9种生物碱和2种酚苷。通过光谱数据分析并与文献中的数据进行比较，确定它们的结构分别为格陵兰紫堇碱（1）、小檗碱（2）、原阿片碱（3）、隐品碱（4）、N - 反式 - 阿魏酰章鱼胺（5）、3 - （4 - 羟基 - 3 - 甲氧基苯基） - N - [2 - （4 - 羟基苯基） - 2 - 甲氧基乙基]丙烯酰胺（6）、N - 顺式 - 对香豆酰章鱼胺（7）、N - 反式 - 对香豆酰去甲肾上腺素（8）、N - 顺式 - 阿魏酰章鱼胺（9）、穿心莲内酯（10）和乙酰丁香酮葡萄糖苷（11）。其中，化合物10和11首次从该属植物中分离得到，化合物1、2和5 - 9首次从该物种中分离得到。对所有分离物进行了体外保护PC12细胞系和抗血小板聚集活性的测试。结果表明，化合物5和7在浓度为10 μmol·L⁻¹时具有保护作用，化合物2对凝血酶（THR）、二磷酸腺苷（ADP）和花生四烯酸（AA）诱导的血小板聚集具有抗聚集活性，化合物3对THR诱导的血小板聚集具有抑制作用。
