Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, No.2 Xudong 2nd Rd, Wuhan, 430062, China.
BMC Genet. 2018 Jun 14;19(1):38. doi: 10.1186/s12863-018-0614-y.
As an important oil crop, growth habit of sesame (Sesamum indicum L.) is naturally indeterminate, which brings about asynchronous maturity of capsules and causes loss of yield.
The genetic basis of determinate growth habit in sesame was investigated by classical genetic analysis through multiple populations, results revealed that it was controlled by an unique recessive gene. The genotyping by sequencing (GBS) approach was employed for high-throughput SNP identification and genotyping in the F population, then a high density bin map was constructed, the map was 1086.403 cM in length, which consisted of 1184 bins (13,679 SNPs), with an average of 0.918 cM between adjacent bins. Based on bin mapping in conjunction with SSR markers analysis in targeted region, the novel sesame determinacy gene was mapped on LG09 in a genome region of 41 kb.
This study dissected genetic basis of determinate growth habit in sesame, constructed a new high-density bin map and mapped a novel determinacy gene. Results of this study demonstrate that we employed an optimized approach to get fine-accuracy, high-resolution and high-efficiency mapping result in sesame. The findings provided important foundation for sesame determinacy gene cloning and were expected to be applied in breeding for cultivars suited to mechanized production.
芝麻（Sesamum indicum L.）作为一种重要的油料作物，其自然生长习性为不定型，导致蒴果成熟期不一致，造成产量损失。
通过对多个群体进行经典遗传分析，研究了芝麻定型生长习性的遗传基础，结果表明其由一个独特的隐性基因控制。在 F 群体中，利用测序基因分型（GBS）方法进行高通量 SNP 鉴定和分型，然后构建了高密度 bin 图谱，图谱全长 1086.403cM，由 1184 个 bin（13679 个 SNPs）组成，相邻 bin 之间的平均距离为 0.918cM。基于 bin 作图和目标区域 SSR 标记分析，将新的芝麻定型基因定位在 LG09 上一个 41kb 的基因组区域。
本研究解析了芝麻定型生长习性的遗传基础，构建了新的高密度 bin 图谱，并定位了一个新的定型基因。本研究结果表明，我们采用了优化的方法，在芝麻中获得了精细、高分辨率和高效的作图结果。这些发现为芝麻定型基因的克隆提供了重要基础，并有望应用于机械化生产所需品种的选育。
