[运动对老年肥胖大鼠脂联素的影响]

[Effect of exercise on adiponectin in aged obese rats].

作者信息

Su Min, Bai You-Ping, Song Wei-Wei, Wang Ming, Shen Rui-Rui, Du Kang-Jian, Xia Xing-Quan, Nie Liu-Wang

机构信息

College of Physical Education, Anhui Normal University, Wuhu 241003, China.

The provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, Anhui Normal University, Wuhu 241003, China.

出版信息

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2018 Apr 8;34(4):345-349. doi: 10.12047/j.cjap.5660.2018.079.

DOI:10.12047/j.cjap.5660.2018.079

PMID:30788943

Abstract

OBJECTIVE

To explore effects of exercise on the expression of adiponectin mRNA and protein in visceral adipose tissue, plasma adiponectin concentration, and insulin resistance of aged obese rats.

METHODS

Male SD rats age to 21 days old were fed with high-fat diet (fat percentage was 36.3% to 40.0%) for three stages of adolescence, maturity and old age to establish elderly obesity rats model. When the rats aged to 60 weeks old, natural growing elderly rats were randomly divided into control group (C) and aged exercise group (AE), =6; elderly obesity rats were randomly divided into obesity control group (OC) and obesity exercise group (OE), =6. The treadmill grade was 0°, the exercise speed and time were 15 m/min×15 min, 4 groups each time, between consecutive groups the rats had 5 minutes rest, the rats were exercised for 60 minutes every day, five days a week, continuous exercise for 8 weeks. Then, the expressions of adiponectin mRNA and protein in visceral adipose tissue were determined. The concentrations of blood glucose, plasma adiponectin and insulin were measured. Insulin resistance was calculated.

RESULTS

Comparison with control group, the expressions of adiponectin mRNA and protein were obviously decreased, the concentration of blood glucose and insulin resistance were significantly increased in obesity control group, while the expressions of adiponectin mRNA and protein were obviously increased. Comparison with obesity control group, the expressions of adiponectin mRNA and protein, the concentration of plasma adiponectin were obviously increased, the concentration of blood glucose and insulin resistance were significantly decreased in obesity exercise group.

CONCLUSIONS

Adiponectin mRNA and protein expression in visceral adipose tissue is decreased and accompanied by high blood glucose and insulin resistance in elderly obesity rats. Exercise can increase the adiponectin mRNA and protein expression in visceral adipose tissue, elevate levels of plasma adiponectin, and decrease the level of blood glucose and insulin resistance in elderly obesity rats.

摘要

目的

探讨运动对老年肥胖大鼠内脏脂肪组织中脂联素mRNA和蛋白表达、血浆脂联素浓度及胰岛素抵抗的影响。

方法

将21日龄雄性SD大鼠在青春期、成熟期和老年期三个阶段喂以高脂饮食（脂肪百分比为36.3%至40.0%）以建立老年肥胖大鼠模型。当大鼠60周龄时，将自然生长的老年大鼠随机分为对照组（C）和老年运动组（AE），每组6只；老年肥胖大鼠随机分为肥胖对照组（OC）和肥胖运动组（OE），每组6只。跑步机坡度为0°，运动速度和时间为15米/分钟×15分钟，每次4组，连续两组之间大鼠休息5分钟，大鼠每天运动60分钟，每周5天，持续运动8周。然后，测定内脏脂肪组织中脂联素mRNA和蛋白的表达。测量血糖、血浆脂联素和胰岛素的浓度。计算胰岛素抵抗。