Lin Chan, Bai Youping, Sun Mingming, Zhang Haoyue, Su Min, Cui Jiaqin, Song Weiwei, Wu Lin
College of Physical Education, Anhui Normal University, Wuhu 241003, China.
Wei Sheng Yan Jiu. 2017 Jan;46(1):70-77.
To investigate effects of different intensity exercise prescription on visceral adipose tissue-derived serine protease inhibitor( Vaspin) mRNA and protein expression in visceral adipose tissue and plasma Vaspin concentration of elderly obese rats.
SD rats as three stage of fattening divided the growth period, middle-aged and elderly, to establish rats model of elderly obesity, 6 natural growing aged rats were selected as blank control group, 18 elderly obese rats were randomly divided into 3 groups, 6 rats in each group: obese control group, low intensity exercise group, moderate intensity exercise group. Exercise prescription were 12 m /min × 15 min for each group, 4groups / times, rest between groups 5min, 60 min/time. 15 m/min×15 min for each group, 4 groups / times, rest between groups 5min, 60 min / time. Vaspin mRNA expression was measured by qRT-PCR and Vaspin protein expression was measured by WB in visceral adipose tissue and plasma Vaspin concentration was measured by ELISA after exercise.
After exercise intervention, (1) Vaspin mRNA(×103) expression:Obese control group(25. 761±11. 581) was higher than the control group(12. 161±5. 648). (P<0. 01), low intensity exercise group and moderate intensity exercise group((10. 482±2. 668), (13. 478±5. 115)) were lower than obese control group (P<0. 01), moderate intensity exercise group was higher than low intensity exercise group, but the difference was not statistically significant (P>0. 05). (2) Vaspin protein expression: Obese control group was higher than the control group (P<0. 01), low intensity exercise group was lower than obese control group (P<0. 05), but moderate intensity exercise group was higher than obese control group (P<0. 01). (3) Plasma Vaspin concentration: Obese control group((25. 000±3. 767) ng/mL) was higher than the control group((23. 765±3. 045) ng/m L), low intensity exercise group and moderate intensity exercise group((25. 637±5. 240) ng/mL and (26. 422±3. 4175) ng/mL) was higher than obese control group, but the difference were not statistically significant( P >0. 05). ( 4) Blood glucose and insulin sensitivity index: Blood glucose of obese control group was higher than the control group( P < 0. 01). Insulin sensitivity index of obese control group was lower than the control group, but the difference was not statistically significant( P > 0. 05). Blood glucose of low and moderate intensity exercise groups were lower than obese control group( P < 0. 05), but insulin sensitivity index of low and moderate intensity exercise groups were higher than obese control group( P < 0. 01).
Vaspin mRNA and protein expression in visceral adipose tissue and plasma Vaspin concentration of elderly obese rats are higher. Low intensity exercise can reduce Vaspin mRNA and protein expression, but increase exercise intensity may improve Vaspin mRNA and protein expression in visceral adipose tissue and plasma Vaspin concentration of elderly obese rats.
探讨不同强度运动处方对老年肥胖大鼠内脏脂肪组织源性丝氨酸蛋白酶抑制剂（Vaspin）mRNA和蛋白表达以及血浆Vaspin浓度的影响。
将SD大鼠按育肥的三个阶段分为生长期、中年期和老年期，建立老年肥胖大鼠模型，选取6只自然生长的老年大鼠作为空白对照组，将18只老年肥胖大鼠随机分为3组，每组6只：肥胖对照组、低强度运动组、中等强度运动组。运动处方分别为每组12m/min×15min，4组/次，组间休息5min，每次60min。每组15m/min×15min，4组/次，组间休息5min，每次60min。运动后采用qRT-PCR检测内脏脂肪组织中Vaspin mRNA表达，采用WB检测Vaspin蛋白表达，采用ELISA检测血浆Vaspin浓度。
运动干预后，（1）Vaspin mRNA（×103）表达：肥胖对照组（2５.761±11.581）高于对照组（12.161±5.648）（P<0.01），低强度运动组和中等强度运动组（（10.482±2.668），（13.478±5.115））低于肥胖对照组（P<0.01），中等强度运动组高于低强度运动组，但差异无统计学意义（P>0.05）。（2）Vaspin蛋白表达：肥胖对照组高于对照组（P<0.01），低强度运动组低于肥胖对照组（P<0.05），但中等强度运动组高于肥胖对照组（P<0.01）。（3）血浆Vaspin浓度：肥胖对照组（（25.000±3.767）ng/mL）高于对照组（（23.765±3.045）ng/mL），低强度运动组和中等强度运动组（（25.637±5.240）ng/mL和（26.422±3.4175）ng/mL）高于肥胖对照组，但差异无统计学意义（P>0.05）。（4）血糖和胰岛素敏感性指数：肥胖对照组血糖高于对照组（P<0.01）。肥胖对照组胰岛素敏感性指数低于对照组，但差异无统计学意义（P>0.05）。低强度和中等强度运动组血糖低于肥胖对照组（P<0.05），但低强度和中等强度运动组胰岛素敏感性指数高于肥胖对照组（P<0.01）。
老年肥胖大鼠内脏脂肪组织中Vaspin mRNA和蛋白表达以及血浆Vaspin浓度较高。低强度运动可降低Vaspin mRNA和蛋白表达，但增加运动强度可能改善老年肥胖大鼠内脏脂肪组织中Vaspin mRNA和蛋白表达以及血浆Vaspin浓度。
