Li Bingbing, Liu Chuncheng, Liu Liang, Li Shuangshu, Fan Nailai, Hou Haiyan, Jin Jing, Xing Yadong
Huai'an Center for Disease Control and Prevention, Huai'an 223001, China.
Wei Sheng Yan Jiu. 2018 Mar;47(2):260-300.
To analyze the prevalence and etiologic agent of Salmonella in livestock and poultry meats, so as to provide data support for controlling contamination and effectively tracing of Salmonella.
Livestock and poultry meats from 8 counties in Huaian from 2015 to 2016 were collected and analyzed for the occurrence of Salmonella according national testing standards. Then the serotype distribution, antimicrobial resistance, virulence gene and molecular subtyping traits of isolated strains were characterized.
Of the total 368 samples, 37( 10. 05%) tested positive for Salmonella. And the positive rate of chicken, duck, pork, beef and mutton were 14. 14%( 14/99), 11. 11%( 10/90), 10. 34%( 12/116) and 1. 59%( 1/63), respectively. 37 Salmonella isolates were separated into 8 serotypes and the main serotypes wereSalmonella enteritidis and Salmonella typhimurium. The antimicrobial resistance test indicated Salmonella isolates showed highest resistance to ampicillin and nalidixic acid( 72. 97%), showed lowest resistance to cefoxitin( 8. 11%). And the resistance phenotypes of Salmonella isolates from different samples and serotypes were different. The carriage rate of inv H and sop E were highest in Salmonella enteritidis and the highest rates of inv H, sop E and rhu M in Salmonella typhimurium. PFGE showed that the pattern similarity of all Salmonella enteritidis was more than 81. 56% and the relationship between the strains with the same source was closer. PFGE pattern of Salmonella typhimurium were different.
The contamination with Salmonella of livestock and poultry meats in Huaian was serious, and the main serotypes were Salmonella enteritidis and Salmonella typhimurium. Salmonella isolates showed high drug resistance and high carriage rate of virulence genes.
分析畜禽肉中沙门氏菌的污染状况及病原，为控制沙门氏菌污染及有效溯源提供数据支持。
采集2015 - 2016年淮安市8个县区的畜禽肉，按照国家检测标准分析沙门氏菌的检出情况。然后对分离菌株的血清型分布、耐药性、毒力基因及分子分型特征进行鉴定。
368份样本中，37份（10.05%）沙门氏菌检测呈阳性。鸡、鸭、猪肉、牛肉和羊肉的阳性率分别为14.14%（14/99）、11.11%（10/90）、10.34%（12/116）和1.59%（1/63）。37株沙门氏菌分离株分为8个血清型，主要血清型为肠炎沙门氏菌和鼠伤寒沙门氏菌。药敏试验表明，沙门氏菌分离株对氨苄西林和萘啶酸耐药率最高（72.97%），对头孢西丁耐药率最低（8.11%）。不同样本和血清型的沙门氏菌分离株耐药表型不同。肠炎沙门氏菌中invH和sopE携带率最高，鼠伤寒沙门氏菌中invH、sopE和rhuM携带率最高。脉冲场凝胶电泳（PFGE）结果显示，所有肠炎沙门氏菌的图谱相似度均大于81.56%，同源菌株间关系更密切。鼠伤寒沙门氏菌的PFGE图谱不同。
淮安市畜禽肉沙门氏菌污染严重，主要血清型为肠炎沙门氏菌和鼠伤寒沙门氏菌。沙门氏菌分离株耐药性高且毒力基因携带率高。
