Jiao Yanna, Ding Li, Zhu Shaohua, Fu Shanliang, Gong Qiang, Li Hui, Wang Libing
Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau, Changsha 410004, China.
Se Pu. 2013 Jan;31(1):83-7. doi: 10.3724/sp.j.1123.2012.08050.
A method for the determination of fluorescent whitening agents in plastic food contact materials by high performance liquid chromatography (HPLC) with fluorescence detector was developed. The samples were extracted with trichloromethane by sonication for 30 min at 40 degrees C. The HPLC method was performed on a column of Eclipse XDB-C18 (250 mm x 4.6 mm, 5 microm) by gradient elution using 5 mmol/L ammonium acetate and acetonitrile as the mobile phases, and detected by the fluorescence detector at an excitation wavelength of 350 nm and an emission wavelength of 430 nm. The experimental results indicated that the four fluorescent whitening agents were separated well. The limits of detection (LOD) (S/N = 3) were 0.3, 0.1, 0.05, 0.14 mg/L, and the limits of quantification (LOQ) (S/N = 10) were 1.0, 0.4, 0.2, 0.5 mg/L for 1,4-bis (4-cyanostyryl) benzene (C. I. 199), 1,4-bis (2-benzoxazolyl) naphthalene (C. I. 367), 4,4'-bis(2-methoxystyryl) biphenyl (C. I. 378) and 2,5-thiophenediylbis (5-tert-butyl-1,3-benzoxazole) (C. I. 184), respectively. Good linearities with correlation coefficients (r2) not less than 0.991 were obtained. The proposed method is simple, accurate, sensitive and can meet the requirements of the routine determination of fluorescent whitening agents in entry-exit products.
建立了一种采用高效液相色谱（HPLC）-荧光检测器测定塑料食品接触材料中荧光增白剂的方法。样品用三氯甲烷在40℃超声提取30分钟。HPLC法采用Eclipse XDB-C18柱（250mm×4.6mm，5μm），以5mmol/L醋酸铵和乙腈为流动相进行梯度洗脱，荧光检测器在激发波长350nm和发射波长430nm下进行检测。实验结果表明，4种荧光增白剂分离良好。1,4-双（4-氰基苯乙烯基）苯（C.I.199）、1,4-双（2-苯并恶唑基）萘（C.I.367）、4,4'-双（2-甲氧基苯乙烯基）联苯（C.I.378）和2,5-噻吩二基双（5-叔丁基-1,3-苯并恶唑）（C.I.184）的检测限（S/N = 3）分别为0.3、0.1、0.05、0.14mg/L，定量限（S/N = 10）分别为1.0、0.4、0.2、0.5mg/L。获得了相关系数（r2）不小于0.991的良好线性关系。该方法简便、准确、灵敏，能够满足出入境产品中荧光增白剂常规测定的要求。
