红细胞储存期间的溶血途径及供体间红细胞形态的变异性
Hemolysis Pathways during Storage of Erythrocytes and Inter-Donor Variability in Erythrocyte Morphology.
作者信息
Melzak Kathryn A, Spouge John L, Boecker Clemens, Kirschhöfer Frank, Brenner-Weiss Gerald, Bieback Karen
机构信息
Institute of Functional Interfaces, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany.
National Center for Biotechnology Information, National Institutes of Health USA, Bethesda, Maryland, USA.
出版信息
Transfus Med Hemother. 2021 Feb;48(1):39-47. doi: 10.1159/000508711. Epub 2020 Jul 16.
BACKGROUND
Red blood cells (RBCs) stored for transfusions can lyse over the course of the storage period. The lysis is traditionally assumed to occur via the formation of spiculated echinocyte forms, so that cells that appear smoother are assumed to have better storage quality. We investigate this hypothesis by comparing the morphological distribution to the hemolysis for samples from different donors.
METHODS
Red cell concentrates were obtained from a regional blood bank quality control laboratory. Out of 636 units processed by the laboratory, we obtained 26 high hemolysis units and 24 low hemolysis units for assessment of RBC morphology. The association between the morphology and the hemolysis was tested with the Wilcoxon-Mann-Whitney U test.
RESULTS
Samples with high stomatocyte counts ( = 0.0012) were associated with increased hemolysis, implying that cells can lyse via the formation of stomatocytes.
CONCLUSION
RBCs can lyse without significant echinocyte formation. Lower degrees of spiculation are not a good indicator of low hemolysis when RBCs from different donors are compared.
背景
用于输血储存的红细胞（RBCs）在储存过程中可能会发生溶血。传统上认为溶血是通过形成棘状红细胞形态发生的，因此，看起来更光滑的细胞被认为具有更好的储存质量。我们通过比较来自不同供体样本的形态分布与溶血情况来研究这一假设。
方法
从一个地区血库质量控制实验室获取红细胞浓缩物。在该实验室处理的636个单位中，我们获得了26个高溶血单位和24个低溶血单位用于评估红细胞形态。形态与溶血之间的关联用Wilcoxon-Mann-Whitney U检验进行测试。
结果
口形红细胞计数高的样本（=0.0012）与溶血增加有关，这意味着细胞可通过口形红细胞的形成而发生溶血。
结论
红细胞可在无明显棘状红细胞形成的情况下发生溶血。当比较来自不同供体的红细胞时，较低程度的棘状化并不是低溶血的良好指标。
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