3B's Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine , Avepark-Parque de Ciência e Tecnologia , 4805-017 Barco , Guimarães , Portugal.
ICVS/3B's-PT Government Associate Laboratory , 4710-057 Braga/Guimarães , Portugal.
ACS Appl Mater Interfaces. 2018 Jul 11;10(27):23311-23320. doi: 10.1021/acsami.8b06154. Epub 2018 Jun 28.
The present work has explored bioactive glass nanoparticles (BGNPs) and developed strontium-doped nanoparticles (BGNPsSr), envisioning orthopedic strategies compatible with vascularization. The nanoparticles were synthesized by the sol-gel method, achieving a diameter of 55 nm for BGNPs and 75 nm for BGNPsSr, and the inclusion of strontium caused no structural alteration. The nanoparticles exhibited high cytocompatibility for human umbilical vein endothelial cells (HUVECs) and SaOS-2. Additionally, the incorporation of strontium emphasized the tubule networking behavior of HUVECs. Our results demonstrate that the nanoparticle dissolution products encouraged the osteogenic differentiation of human adipose stem cells as it favored the expression of key genes and proteins associated with osteogenic lineage. This effect was markedly enhanced for BGNPsSr, which could prompt stem cell osteogenic differentiation without the typical osteogenic inducers. This study not only supports the hypothesis that BGNPs might play a significant role in osteogenic commitment but also highlights that the designed BGNPsSr is a valuable tool for stem cell "tune-up" in bone tissue engineering applications.
本研究探索了生物活性玻璃纳米颗粒（BGNPs）并开发了掺锶的纳米颗粒（BGNPsSr），旨在为血管化提供与骨科策略相兼容的方法。通过溶胶-凝胶法合成了纳米颗粒，BGNPs 的直径为 55nm，BGNPsSr 的直径为 75nm，且锶的掺入并未引起结构改变。纳米颗粒对人脐静脉内皮细胞（HUVECs）和 SaOS-2 具有高细胞相容性。此外，锶的掺入强调了 HUVECs 的管状网络行为。我们的结果表明，纳米颗粒溶解产物促进了人脂肪干细胞的成骨分化，因为它有利于与成骨谱系相关的关键基因和蛋白的表达。BGNPsSr 的效果更为显著，它可以在没有典型成骨诱导剂的情况下促进干细胞的成骨分化。这项研究不仅支持了 BGNPs 可能在成骨分化中发挥重要作用的假设，还强调了设计的 BGNPsSr 是骨组织工程应用中干细胞“调整”的有价值工具。
