National Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China.
National Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China.
Food Chem Toxicol. 2018 Sep;119:6-13. doi: 10.1016/j.fct.2018.06.018. Epub 2018 Jun 12.
Acteoside has been reported to have antioxidant and neuroprotective effect, which is a promising therapeutic way in prevention and treatment of Parkinson's disease. The present study was aimed to understand the neuroprotective effect of acteoside and to elucidate its underlying mechanism. 6-hydroxydopamine (6-OHDA)-induced neural damage in zebrafish model was used to study the protective effect of acteoside on Parkinson's disease (PD). Locomotion behavioral test showed that acteoside could prevent 6-OHDA-stimulated movement disorders. Anti-tyrosine hydroxylase (TH) whole-mount immunostaining analysis showed that acteoside could prevent 6-OHDA-induced dopaminergic neuron death. In addition, pretreatment with acteoside could upregulate antioxidative enzymes by activating the Nrf2/ARE signaling pathway in zebrafish. Meanwhile, acteoside was found to be distributed in the brain after intraperitoneal injection into the adult zebrafish, indicating that this compound could penetrate the blood-brain-barrier (BBB). This study demonstrated that acteoside could penetrate BBB and have potential therapeutic value for PD by activating the Nrf2/ARE signaling pathway and attenuating the oxidative stress.
毛蕊花糖苷具有抗氧化和神经保护作用，是预防和治疗帕金森病的一种很有前途的治疗方法。本研究旨在了解毛蕊花糖苷的神经保护作用，并阐明其潜在的机制。使用 6-羟多巴胺（6-OHDA）诱导的斑马鱼模型来研究毛蕊花糖苷对帕金森病（PD）的保护作用。运动行为测试表明，毛蕊花糖苷可以预防 6-OHDA 刺激引起的运动障碍。抗酪氨酸羟化酶（TH）全组织免疫染色分析表明，毛蕊花糖苷可以预防 6-OHDA 诱导的多巴胺能神经元死亡。此外，毛蕊花糖苷预处理可以通过激活 Nrf2/ARE 信号通路来上调抗氧化酶。同时，发现毛蕊花糖苷在腹腔注射到成年斑马鱼后分布在大脑中，表明该化合物可以穿透血脑屏障（BBB）。这项研究表明，毛蕊花糖苷可以通过激活 Nrf2/ARE 信号通路和减轻氧化应激来穿透血脑屏障，并具有治疗 PD 的潜在价值。
