Department of Physics, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, South Korea.
KI for Health Science and Technology, KAIST, Daejeon, 34141, Republic of Korea.
Sci Rep. 2018 Jun 15;8(1):9192. doi: 10.1038/s41598-018-25886-8.
Unlike mammalian erythrocytes, amphibian erythrocytes have distinct morphological features including large cell sizes and the presence of nuclei. The sizes of the cytoplasm and nuclei of erythrocytes vary significantly over different species, their environments, or pathophysiology, which makes hematological studies important for investigating amphibian species. Here, we present a label-free three-dimensional optical quantification of individual amphibian erythrocytes from frogs Pelophylax nigromaculatus (Rana nigromaculata). Using optical diffraction tomography, we measured three-dimensional refractive index (RI) tomograms of the cells, which clearly distinguished the cytoplasm and nuclei of the erythrocytes. From the measured RI tomograms, we extracted the relevant biochemical parameters of the cells, including hemoglobin contents and hemoglobin concentrations. Furthermore, we measured dynamic membrane fluctuations and investigated the mechanical properties of the cell membrane. From the statistical and correlative analysis of these retrieved parameters, we investigated interspecific differences between frogs and previously studied mammals.
与哺乳动物的红细胞不同，两栖动物的红细胞具有独特的形态特征，包括较大的细胞尺寸和核的存在。红细胞的细胞质和细胞核的大小在不同的物种、环境或病理生理学中差异显著，这使得血液学研究对于研究两栖动物物种非常重要。在这里，我们提出了一种无标记的三维光学定量方法，可以从青蛙 Pelophylax nigromaculatus（黑斑蛙）中获得单个两栖动物红细胞。我们使用光学衍射层析技术测量了细胞的三维折射率（RI）断层图像，这些图像清楚地区分了红细胞的细胞质和细胞核。从测量的 RI 断层图像中，我们提取了细胞的相关生化参数，包括血红蛋白含量和血红蛋白浓度。此外，我们还测量了细胞膜的动态波动，并研究了细胞膜的力学性质。通过对这些检索参数的统计和相关性分析，我们研究了蛙类与之前研究的哺乳动物之间的种间差异。
