相关三维荧光和折射率断层扫描：弥合分子特异性与定量生物成像之间的差距。
Correlative three-dimensional fluorescence and refractive index tomography: bridging the gap between molecular specificity and quantitative bioimaging.
作者信息
Kim Kyoohyun, Park Wei Sun, Na Sangchan, Kim Sangbum, Kim Taehong, Heo Won Do, Park YongKeun
机构信息
Department of Physics, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, South Korea.
KI for Health Science and Technology (KIHST), KAIST, Daejeon 34141, South Korea.
出版信息
Biomed Opt Express. 2017 Nov 17;8(12):5688-5697. doi: 10.1364/BOE.8.005688. eCollection 2017 Dec 1.
Abstract
Optical diffraction tomography (ODT) provides label-free three-dimensional (3D) refractive index (RI) measurement of biological samples. However, due to the nature of the RI values of biological specimens, ODT has limited access to molecular specific information. Here, we present an optical setup combining ODT with three-channel 3D fluorescence microscopy, to enhance the molecular specificity of the 3D RI measurement. The 3D RI distribution and 3D deconvoluted fluorescence images of HeLa cells and NIH-3T3 cells are measured, and the cross-correlative analysis between RI and fluorescence of live cells are presented.
摘要
光学衍射层析成像（ODT）可对生物样品进行无标记的三维（3D）折射率（RI）测量。然而，由于生物样本折射率值的特性，ODT获取分子特异性信息的能力有限。在此，我们展示了一种将ODT与三通道3D荧光显微镜相结合的光学装置，以增强3D RI测量的分子特异性。我们测量了HeLa细胞和NIH-3T3细胞的3D RI分布和3D去卷积荧光图像，并给出了活细胞RI与荧光之间的互相关分析。
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