Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid Wastes, National Laboratory of Mineral Materials, School of Materials Science and Technology, China University of Geosciences, Beijing, 100083, P. R. China.
Chemistry. 2018 Sep 18;24(52):13830-13838. doi: 10.1002/chem.201801884. Epub 2018 Aug 19.
The development of well-controlled drug carriers that are stable and highly effective for the delivery of anticancer agents is challenging. Herein, we report a novel pH-controlled drug delivery system, utilizing reducing graphene oxide (rGO)-polymer self-assembly films as carriers, for the preparation of effective drug nanorods and nanoparticles. In this system, the rGO-polymer carriers were constructed by the alternating assembly of poly-l-lysine (PLL) and polyaspartic acid (PASP) around the rGO sheets. Furthermore, the rGO-polymer cores, which possess a positively charged surface as the desired template, could assemble with negatively charged doxorubicin (DOX) via electrostatic interactions. The DOX embedding efficiency and the morphology of the drug nanocomposites could be controlled by the number of rGO-polymer bilayers and concentration of the rGO-polymer bilayers and the initial DOX concentration. Importantly, the release of DOX could be regulated by controlling the pH and by using a NIR laser. Under acidic conditions, the interactions between the PASP layer and DOX molecules can be broken, resulting in gradual release of the DOX molecules. Upon NIR irradiation, the release of DOX could be further accelerated and a photothermal effect from rGO induced. Cellular uptake and cytotoxicity experiments indicate that the drug nanocomposites possess effective anticancer activity. Thus, in this work, we present a useful strategy for the fabrication of pH-responsive drug nanocomposites for combined photothermal and chemical therapy. The nanocomposite can be used as a potential drug delivery system for practical cancer treatment.
开发能够稳定且高效传递抗癌药物的可控药物载体具有挑战性。在此，我们报告了一种新型 pH 控制药物输送系统，利用还原氧化石墨烯 (rGO)-聚合物自组装膜作为载体，用于制备有效的药物纳米棒和纳米颗粒。在该系统中，rGO-聚合物载体是通过在 rGO 片周围交替组装聚-l-赖氨酸 (PLL) 和聚天冬氨酸 (PASP) 构建的。此外，rGO-聚合物核具有正电荷表面，可作为所需模板与带负电荷的阿霉素 (DOX) 通过静电相互作用组装。通过控制 rGO-聚合物双层的数量和 rGO-聚合物双层和初始 DOX 浓度，可以控制 DOX 的包埋效率和药物纳米复合材料的形态。重要的是，通过控制 pH 值和使用近红外激光可以调节 DOX 的释放。在酸性条件下，PASP 层与 DOX 分子之间的相互作用可以被打破，导致 DOX 分子逐渐释放。近红外照射后，DOX 的释放可以进一步加速，并产生 rGO 诱导的光热效应。细胞摄取和细胞毒性实验表明，药物纳米复合材料具有有效的抗癌活性。因此，在这项工作中，我们提出了一种用于制备 pH 响应性药物纳米复合材料的有用策略，用于联合光热和化学治疗。该纳米复合材料可用作实际癌症治疗的潜在药物输送系统。
