Allan M, Montague P, Grindlay G J, Sibbet G, Donovan-Peluso M, Bank A, Paul J
Nucleic Acids Res. 1985 Sep 11;13(17):6125-36. doi: 10.1093/nar/13.17.6125.
We have introduced a plasmid containing the human epsilon-globin gene either stably or transiently into a number of erythroid or non-erythroid cell lines, and analysed the accuracy and efficiency of transcription. In non-erythroid cells (or in mouse erythroleukaemia (MEL) cells in which adult but not embryonic globin genes are expressed) transcription of the epsilon-globin gene occurs mainly from a site 200 bp upstream of the major cap site (the -200 cap site). In the human K562 cell line, in which the endogenous epsilon-globin gene is transcribed at high levels, transcription initiation from the introduced gene occurs mainly from the major cap site. Transcriptional activity of the epsilon-globin gene introduced into K562 cell is quantitatively similar to that of the endogenous gene. This suggests the presence (or absence) in K562 cells of factor(s) which activate (or repress) the epsilon-globin gene in a tissue specific manner.
我们已将含有人类ε-珠蛋白基因的质粒稳定或瞬时导入多种红系或非红系细胞系，并分析了转录的准确性和效率。在非红系细胞中（或在表达成人而非胚胎珠蛋白基因的小鼠红白血病（MEL）细胞中），ε-珠蛋白基因的转录主要发生在主要帽位点上游200 bp处的一个位点（-200帽位点）。在人类K562细胞系中，内源性ε-珠蛋白基因高水平转录，导入基因的转录起始主要发生在主要帽位点。导入K562细胞的ε-珠蛋白基因的转录活性在数量上与内源性基因相似。这表明K562细胞中存在（或不存在）以组织特异性方式激活（或抑制）ε-珠蛋白基因的因子。
