Krogerus Leena, Kholová Ivana
Department of Pathology, HUSLAB, Jorvi Hospital, Espoo, Finland.
Department of Pathology, Fimlab Laboratories, Tampere University Hospital, Tampere, Finland.
Acta Cytol. 2018;62(4):237-243. doi: 10.1159/000489769. Epub 2018 Jun 15.
The cell block (CB) technique refers to the processing of sediments, blood clots, or grossly visible tissue fragments from cytological specimens into paraffin blocks that can be cut and stained by the same methods used for histopathology. The technique brings additional tissue architectural information. CB can be used for ancillary techniques such as immunocytochemistry and molecular techniques.
We reviewed the literature on the various preparatory techniques of CBs.
There is a wide range of preparatory techniques for CBs and no golden standard for CBs exists: tens of methods are used in various institutions. The majority of the methods are modified in house techniques with a few commercially available kits. The techniques most commonly used are the plasma/thrombin method, the agar method, and commercially available Histogel- and Cellient CB-methods. Dissatisfaction with the cellular yield of the CBs is common.
In the CBs, the cytological material is preserved for future use, which is a tremendous advantage in the era of targeted therapy and biobanking. The CB is thus central to the future of cytology: more can be done with less material and with less invasiveness to the patient.
细胞块（CB）技术是指将细胞学标本中的沉淀物、血凝块或肉眼可见的组织碎片处理成石蜡块，这些石蜡块可以通过用于组织病理学的相同方法进行切片和染色。该技术能提供额外的组织结构信息。CB可用于免疫细胞化学和分子技术等辅助技术。
我们回顾了关于CB各种制备技术的文献。
CB有多种制备技术，且不存在CB的金标准：不同机构使用数十种方法。大多数方法是内部改良技术，只有少数商业可用试剂盒。最常用的技术是血浆/凝血酶法、琼脂法以及商业可用的Histogel和Cellient CB法。对CB细胞产量不满意很常见。
在CB中，细胞学材料得以保存以备将来使用，这在靶向治疗和生物样本库时代是一个巨大优势。因此，CB对细胞学的未来至关重要：用更少的材料、对患者更少的侵入性就能做更多的事情。
