Hammack Audrey, Rihani Rashed T, Black Bryan J, Pancrazio Joseph J, Gnade Bruce E
Department of Chemistry, University of Texas at Dallas, Richardson, TX, 75080, USA.
Department of Bioengineering, University of Texas at Dallas, Richardson, TX, 75080, USA.
Biomed Microdevices. 2018 Jun 16;20(2):48. doi: 10.1007/s10544-018-0295-3.
Substrate-integrated microelectrode arrays (MEAs) are non-invasive platforms for recording supra-threshold signals, i.e. action potentials or spikes, from a variety of cultured electrically active cells, and are useful for pharmacological and toxicological studies. However, the MEA substrate, which is often fabricated using semiconductor processing technology, presents some challenges to the user. Specifically, the electrode encapsulation, which may consist of a variety of inorganic and organic materials, requires a specific substrate preparation protocol to optimize cell adhesion to the surface. Often, these protocols differ from and are more complex than traditional protocols for in vitro cell culture in polystyrene petri dishes. Here, we describe the fabrication of an MEA with indium tin oxide microelectrodes and a patterned polystyrene electrode encapsulation. We demonstrate the electrochemical stability of the electrodes and encapsulation, and show viable cell culture and in vitro recordings.
衬底集成微电极阵列（MEA）是用于记录来自多种培养的电活性细胞的阈上信号（即动作电位或尖峰）的非侵入性平台，对药理学和毒理学研究很有用。然而，通常使用半导体加工技术制造的MEA衬底给用户带来了一些挑战。具体而言，电极封装可能由多种无机和有机材料组成，需要特定的衬底制备方案来优化细胞对表面的粘附。通常，这些方案不同于且比在聚苯乙烯培养皿中进行体外细胞培养的传统方案更复杂。在此，我们描述了一种带有氧化铟锡微电极和图案化聚苯乙烯电极封装的MEA的制造方法。我们展示了电极和封装的电化学稳定性，并展示了可行的细胞培养和体外记录。
