甲基弯曲菌OB3b基因操作的最新进展
Recent Advances in the Genetic Manipulation of Methylosinus trichosporium OB3b.
作者信息
Ro Soo Y, Rosenzweig Amy C
机构信息
Northwestern University, Evanston, IL, United States.
Northwestern University, Evanston, IL, United States.
出版信息
Methods Enzymol. 2018;605:335-349. doi: 10.1016/bs.mie.2018.02.011. Epub 2018 Apr 11.
Abstract
Methanotrophic bacteria utilize methane as their sole carbon and energy source. Studies of the model Type II methanotroph Methylosinus trichosporium OB3b have provided insight into multiple aspects of methanotrophy, including methane assimilation, copper accumulation, and metal-dependent gene expression. Development of genetic tools for chromosomal editing was crucial for advancing these studies. Recent interest in methanotroph metabolic engineering has led to new protocols for genetic manipulation of methanotrophs that are effective and simple to use. We have incorporated these newer molecular tools into existing protocols for Ms. trichosporium OB3b. The modifications include additional shuttle and replicative plasmids as well as improved gene delivery and genotyping. The methods described here render gene editing in Ms. trichosporium OB3b efficient and accessible.
摘要
甲烷营养细菌利用甲烷作为其唯一的碳源和能源。对典型的II型甲烷营养菌甲基弯曲菌OB3b的研究为甲烷营养的多个方面提供了深入了解，包括甲烷同化、铜积累和金属依赖性基因表达。用于染色体编辑的遗传工具的开发对于推进这些研究至关重要。最近对甲烷营养菌代谢工程的兴趣导致了新的甲烷营养菌遗传操作方案，这些方案有效且易于使用。我们已将这些更新的分子工具纳入现有的甲基弯曲菌OB3b实验方案中。这些修改包括额外的穿梭和复制质粒，以及改进的基因传递和基因分型。此处描述的方法使甲基弯曲菌OB3b中的基因编辑高效且易于实现。
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2
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3
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4
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Front Microbiol. 2017 Jan 10;7:2167. doi: 10.3389/fmicb.2016.02167. eCollection 2016.
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