Kriegsmann Jörg, Casadonte Rita, Kriegsmann Katharina, Longuespée Rémi, Kriegsmann Mark
MVZ for Histology, Cytology and Molecular Diagnostics Trier, Trier, Germany; Proteopath GmbH, Trier, Germany.
Proteopath GmbH, Trier, Germany.
Pathol Res Pract. 2018 Aug;214(8):1057-1063. doi: 10.1016/j.prp.2018.05.009. Epub 2018 May 17.
Mass spectrometric (MS) techniques are applied in various areas of medical diagnostics. For the detection of microbiological germs and genetic mutations, MS is a method used in routine. Since MS also allows the analysis of proteins and peptides, it seems an ideal candidate to supplement histopatholological diagnostics. Matrix-assisted laser desorption/ionization time-of-flight Imaging MS links molecular analysis of numerous analytes with morphological information about their spatial distribution in cells or tissues. Herein, we review principle MS techniques as well as potential applications in pathology and discuss our vision for a future workflow.
质谱（MS）技术应用于医学诊断的各个领域。对于微生物病原体和基因突变的检测，MS是一种常规使用的方法。由于MS还能够分析蛋白质和肽，它似乎是补充组织病理学诊断的理想选择。基质辅助激光解吸/电离飞行时间成像质谱将众多分析物的分子分析与其在细胞或组织中的空间分布的形态学信息联系起来。在此，我们综述了主要的MS技术以及在病理学中的潜在应用，并讨论了我们对未来工作流程的展望。
