Hernández-Monjaraz Beatriz, Santiago-Osorio Edelmiro, Ledesma-Martínez Edgar, Alcauter-Zavala Andrés, Mendoza-Núñez Víctor Manuel
1 Research Unit on Gerontology, FES Zaragoza, National Autonomous University of Mexico, Mexico City, Mexico.
2 Hematopoiesis and Leukemia Laboratory, Research Unit on Cell Differentiation and Cancer, FES Zaragoza, National Autonomous University of Mexico, Mexico City, Mexico.
J Int Med Res. 2018 Jul;46(7):2983-2993. doi: 10.1177/0300060518773244. Epub 2018 Jun 18.
Objective To report a case of successful allogeneic grafting of mesenchymal dental pulp stem cells (DPSCs) as preliminary findings in a patient with periodontal disease enrolled into clinical trial ISRCTN12831118. Methods Mesenchymal stem cells from the dental pulp of a deciduous tooth from a 7-year-old donor were separated from the pulp chamber and processed via enzymatic digestion and centrifugation. DPSCs were passaged and cultured on a 35 × 13 mm culture dish in minimum essential medium-alpha, without supplementation. After reaching 80% confluency, 5 x 10 allogeneic DPSCs in 250 µl phosphate buffered saline were seeded onto a dry scaffold of lyophilized collagen-polyvinylpyrrolidone sponge placed in the left lower premolar area of a 61-year-old patient with periodontal disease. Surgical access to the lower premolar area was achieved using the flap technique. Results At 3 and 6 months following allogeneic graft, the patient showed no sign of rejection and exhibited decreases in tooth mobility, periodontal pocket depth and bone defect area. Bone mineral density had increased at the graft site. Conclusions Regenerative periodontal therapy using DPSCs of allogeneic origin may be a promising treatment for periodontal disease-induced bone defects.
目的 报告1例间充质牙髓干细胞（DPSCs）异体移植成功的病例，作为纳入临床试验ISRCTN12831118的一名牙周病患者的初步研究结果。方法 从一名7岁供体的乳牙牙髓中分离间充质干细胞，从牙髓腔中取出并通过酶消化和离心进行处理。DPSCs在35×13mm培养皿中于无添加物的α-最低必需培养基中传代培养。达到80%汇合度后，将250μl磷酸盐缓冲盐水中的5×10个异体DPSCs接种到一名61岁牙周病患者左下前磨牙区放置的冻干胶原-聚乙烯吡咯烷酮海绵干燥支架上。采用翻瓣技术进入下前磨牙区进行手术。结果 在异体移植后3个月和6个月时，患者无排斥迹象，牙齿松动度、牙周袋深度和骨缺损面积均减小。移植部位的骨矿物质密度增加。结论 使用异体来源的DPSCs进行牙周再生治疗可能是治疗牙周病所致骨缺损的一种有前景的方法。
